BME100 f2015:Group17 8amL1

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Contents

OUR TEAM

Name: Jadyn Ziegler
Name: Jadyn Ziegler
Name: Jake Feltz
Name: Jake Feltz
Name: Nathan Natividad
Name: Nathan Natividad
Name: Zachary Ramsey
Name: Zachary Ramsey
Name: Sam Stone
Name: Sam Stone
Name: Mahoro Uwiringiyimana
Name: Mahoro Uwiringiyimana

LAB 1 WRITE-UP

Independent and Dependent Variables

The independent variable is lipopolysaccharide, because this agent will be manipulated to measure the increase of the inflammatory protein.


The dependent variable is the level of the inflammatory protein (Inflammotin), because this is what will be measured after administering the dosage.

Experimental Design

Groups
Thlere will be 11 groups total. One group will be a control group to provide a reference point for the experiment. The other ten groups will all be assigned an amount of lipopolysaccharide ranging from 1 to 10 milligrams. There will be 11 groups total to allow experimenters to test multiple dosages to find a closer minimum amount of lipopolysaccharide.


Age
All test subjects will be 65 to 70 years old due to limited funds and resources. The limited age range will ensure that the results are not skewed due to different body functions that might result in different effects on the lipopolysaccharide dose.


Number of subjects per group
There will be ten subjects per test group. This number was selected to provide a range of data that will account for different body types and abilities to produce protein, thus creating a more accurate and reliable average.





Subject Selection

We will be choosing subjects based upon their age and also their willingness to participate. Due to the fact that we have a very low budget we cannot offer an incentive to our participants in the experiment so as a result we are depending on volunteers only to obtain our data. Subjects in our experiment need to be between the ages of 65 and 70 and in fair health. Once we have a population for our survey, we will then perform an SRS and split the subjects up into 11 groups in order to ensure randomization. The first group will be a control, and the following ten will all be experimental. For each of the experimental groups a certain dosage of the medication will be given to the subjects in the group starting with 10 mg for the first group and decreasing by 1 mg for each remaining group. This selection process will result in a response bias due to the face that we are depending upon volunteers to fill our sample.





Sources of Error and Bias

Sources of Error/Bias

-Location: Different areas may affect various health issues, so testing all subjects in the same area may create bias

-Other medications might affect new pill’s effectiveness: If subjects are on a medication at the time of taking the lipopolysaccharide pill, the different medications may affect one another that produce different results

-Volunteer Bias: People that volunteer may be healthier than people who had not volunteered. With volunteers it is difficult to test a reliable sample

-Unhealthy subjects might affect new pill’s effectiveness due to strong immune response from pill

-Low funding prevents us from replicating the experiment- results from other trials may have been different







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