BME100 f2015:Group13 1030amL2

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Contents

OUR TEAM

Name: Tanner Ivey
Name: Tanner Ivey
Name: Christopher Chen
Name: Christopher Chen
Name: Bramuel Simiyu
Name: Bramuel Simiyu
Name: Drew Worman
Name: Drew Worman
Name: Kaylee Antill
Name: Kaylee Antill
Name: Jonah Brosemann
Name: Jonah Brosemann

LAB 2 WRITE-UP

Descriptive Statistics

Rat Study

0 mg 10 mg
Mean (pg/ml) 10.516 11.112
Standard Deviation (pg/ml) 2.226 7.403
Standard Error (pg/ml) 0.995 3.311


Human Study

0 mg 5 mg 10 mg 15 mg
Mean (pg/ml) 3.834 8.932 61.622 657.941
Standard Deviation (pg/ml) 1.523 1.594 30.111 212.943
Standard Error (pg/ml) 0.482 0.504 9.522 67.338



Results

Rat Study

Rat Experiment Graph

Human Study

Human Experiment Graph




Analysis

Rat Study

We used the t-test to analyze the data from the rats because the rats were divided into two groups, 0mg and 10 mg doses of LPS. The t-test is a statistical test used to investigate differences between two groups. In addition, the t-test was unpaired because the two rat groups were made up of different rats.

t-test value t-test value to achieve significance Significant?
0.867 0.05 no

Human Study

We used the ANOVA to analyze the data from the human study because that study utilized four groups, and the ANOVA is used to analyze differences between more than two groups. After the ANOVA test indicated that there could be a statistically significant difference between the groups, we carried out a post-hoc test to confirm that the differences were actually statistically significant.

Post-hoc Tests t-test value Corrected p-value to achieve significance Significant?
0 mg vs 5 mg 8.596E-7 0.0083 yes
0 mg vs 10 mg 9.944E-6 0.0083 yes
0 mg vs 15 mg 1.394E-8 0.0083 yes
5 mg vs 10 mg 3.019E-5 0.0083 yes
5 mg vs 15 mg 1.571E-8 0.0083 yes
10 mg vs 15 mg 6.482E-8 0.0083 yes




Summary/Discussion

In the human study, administering LPS doses to the subjects was shown to increase Inflammotin protein levels by a statistically significant amount. With a 0 mg LPS dose, Inflammotin levels were at an average of 3.834 pg/ml. This rose to 8.932 pg/ml with a 5 mg dose, 61.622 with a 10 mg dose, and 657.941 pg/ml with a 15 mg dose of LPS. We can conclude with greater than 95% confidence that the LPS increases Inflammotin levels in elderly humans.

In the rat study, however, there was no statistically significant difference between Inflammotin levels between rats given a 0mg dose of LPS and a 10 mg dose. Rats given 0 mg of LPS had an average Inflammotin level of 10.516 pg/ml while rats given a 10 mg dose had an average level of 11.112 pg/ml. We cannot conclude that LPS has an effect on Inflammotin levels in rats from the data provided.

Both studies were designed to determine whether LPS had an effect on Inflammotin protein levels. The human study, however, was much better constructed. A greater range of LPS doses were tested on humans, and each test group was a larger size in comparison to the rat study. This increased the reliability of the results obtained from the human study and we were able to draw a clear conclusion after analyzing the study's data. From the much smaller amount of data in the rat study, we were not able to confirm or deny the idea that LPS has an effect on Inflammotin levels.

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