BME100 f2014:Group21 L2

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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Contents

OUR TEAM

Name: Dustin D. Burley
Name: Dustin D. Burley
Name: Mackenzie Konves
Name: Mackenzie Konves
Name: Scott Boege
Name: Scott Boege
Name: Nicholas Goettl
Name: Nicholas Goettl
Name: Travis Tibbs
Name: Travis Tibbs
Name: Lionel Davis
Name: Lionel Davis

LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1
Will there be an effect of LPS on rats?

There was almost no change in the levels of Inflammotin in the rats. The means of the 0mg group and the 10mg group are 10.516pg/ml and 11.112pg/ml respectively. The standard deviation of the Omg group is 2.225552 and the 10mg group has a 7.402886 standard deviation. Standard error for the 0mg group is 0.995297 and the 10mg group has a standard error of 3.310671.


Effect of LPS on Rats

Experiment 2

Will there be an effect of LPS on humans? MEAN, STANDARD DEVIATION, STANDARD ERROR OF THE EXPERIMENT ON HUMANS

There was a strong positive correlation between the different dosages of LPS and the production of Inflammotin when tested on human subjects. The means for the groups of 0mg, 5mg, 10mg, and 15mg doses are 3.834pg/ml, 8.932pg/ml, 61.622pg/ml, and 657.941pg/ml respectively. Standard deviations for the 0mg, 5mg, 10mg, and 15mg dose groups are 1.152301018, 1.593931547, 30.11069386, and 212.9429 respectively. The standard errors for the 0mg, 5mg, 10mg, and 15mg dose groups are 0.481618106, 0.504045412, 9.552183745, and 67.33848166 respectively.


Effect of LPS on Rats



Results

Experiment 1

Effect of LPS on Rats The T-test for the rat data

The results showed that the the dosage of LPS on rats didn't do much on their levels of inflammotin, as the p-value is very large (0.867403). There could have been an error or outlier in tests done on the rats. Rats could possibly require a dosage of LPS higher than 10mg, or the test conducted was not done properly. It is also possible that LPS has no effect on rats.

Experiment 2
Effect of LPS on Humans The full ANOVA test

The p-value of the one-way ANOVA test was 1E-16. This small p-value means that there is a difference between the levels of inflammotin in the groups. There is a very good chance that no outliers are present and the experiment was conducted properly.



Analysis

Experiment 1
A one-way t-test was chosen for this experiment since only two groups are present. Since the p-value(0.867403) of the t-test is greater than 0.05, we do not have statistical evidence that there is a difference in inflammotin production between the group that received a 0mg dose and the group that received a 10mg dose.


Experiment 2
ANOVA test for human inflammotin

ANOVA test

A one-way ANOVA test was chosen for this experiment in order to investigate the differences among more than two groups. Since the p-value(1E-16) of the ANOVA test is less than 0.05, we can determine that there is a statistical difference in the levels of inflammotin found in humans among those who did not take LPS, those who took 5mg of LPS, those who took 10mg of LPS, and those who took 15mg of LPS.

Summary/Discussion

An experiment was performed on a group of rats and subsequently, a group of elderly people living in a retirement home. From the experiment, it was initially concluded that the dosage of Inflammotin did not affect the protein levels of the rats; the variation between the 0 and 10mg doses was 0.6 pg/ml. A one-way t-test was performed to quantify the differences between the two groups of rats and validate the results. This test proved the study to be inconclusive because the small amount of data showed no quantifiable differences. Unlike the rat experiment, an effect was observed in human subjects; the average production of Inflammotin between the 0, 5, 10, and 15mg dosages of LPS increased exponentially. Furthermore, an ANOVA test was performed to evaluate differences between the four human groups. This test validated the results proving that higher dosages of LPS caused increased production of Inflammotin in humans, as the p-value was less than 0.05. In conclusion, this experiment proved the dosages of LPS affected the production of Inflammotin in humans but proved inconclusive in rats.

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