BME100 f2013:W900 Group16 L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
The independent variable is the dose of the lipopolysaccharide. The dependent variable is the inflammatory protein (Inflammotin) in the elderly.
Our experiment will have eight groups.
We use the twenty-four collective test results (8 groups with 3 sub-tests per group)and perform a ANOVA test. We then look at the p-value of the test to determine whether or not the doses are statistically significant.
ELISA Test http://virology-online.com/general/Test5.htm
In our experiment, our subject selection will be taken randomly from the age group 65 years to 85 years. Experimental subjects must not have medication that includes lipopolysaccharide to keep results from being unintentionally falsified. Any race/ethnicity will be admitted into the experiment to observe if certain ethnic genes affect inflammation protein presence.
Sources of Error and Bias
A potential source of error is the variability between subjects such as gender, ethnicity, diet/exercise, medical conditions, and other medications. To control possible errors and biases, prior to experimentation, each subject will undergo a blood test to document health conditions and initial starting levels of blood content to later be able to compare blood results after the lipopolysaccharide doses. The experiment will be a double blind study to ensure there is no participant or experimenter bias. The patients will not know which dosage they received and patients that take 0mg of lipopolysaccharide will receive placebos. The patients will be observed and the data will be collected by someone other than the experimenter.