BCH4160/2011:Notebook/Laura Lee/2011/10/04

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Contents

Bomb Calorimetry

The purpose of this experiment was to understand the fundamentals of using a bomb calorimeter, and measure a standard curve of temperature change of benzoic acid.

Materials

The materials of this experiment were as follows:

  • Benzoic acid (premade pellets)
  • Oxygen cylinder
  • Isothermal bomb calorimeter
  • 25°C water bath
  • 2L volumetric flask
  • 1mL pipet

Procedure

We used the following procedure: "Bomb calorimetry: The energy concent of pizza" Stout, Roland P.; Nettleton, Faith E.; Price, Lynn M. J. Chem. Educ. 1985, 62, 438-439.

Data/Results

The raw data of our benzoic acid bomb cal.: Image:BOMB CALORIMETRY RAW DATA.xls


Standard Curve of Benzoic Acid Bomb Calorimetry

Image:Bomb Cal. Graph.jpg


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PCR

The purpose of this experiment was to learn some of the basics of polymerase chain reactions (PCR) and gel electrophoresis. This procedure simulates a crime scene investigation and allows us to analyze the genotypes of several DNA samples: one from a hypothetical crime scene and four from hypothetical suspects.


Materials

For a complete materials list refer to:

PCR

  • Suspect DNA (A,B,C,D)
  • Crime scene DNA
  • Thermal cycler

Procedure

The purpose of this procedure to use PCR to amplify DNA from a 'crime scene.'

Protocol is listed as in: "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc.

Go to part 2 for continued protocol.

Data/Results

Go to part 2 for results.

Contact

This experiment was done with High Point University students Jigesh Patel and Brittany Webster.

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