AU Biomaterials Design Lab:Notebook/Quantum Dots/2014/01/31

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Objective

Synthesize Mn-doped ZnS quantum dots using the synthesis described here

Description

Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM

  1. Add 176μL of 5mM Zn(CH3COO)2 to a test tube
  2. Add 24μL of 5mM Mn((CH3COO)2) to the test tube
  3. Add 200μL of 40mM NH4CH3COO to the test tube
  4. Add 244μL of 0.55mg/mL protein to the test tube
  5. Add 316μL of 10mM NH4OH
    1. This should bring the pH up to 8.2. Check on this with a pH strip
  6. Bring the volume up to 2.5mL with water (Add 1540μL of water)
  7. Incubate at room temperature for 1 hour
  8. Add 200μL of 5mM Na2S
    1. When you add this add it drop wise (as best as you can) while vigorously shaking the test tube.
  9. Incubate at 37C for 5 days.

Test Tube 2: Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM

  1. Add 150μL of 5mM Zn(CH3COO)2 to a test tube
  2. Add 50μL of 5mM Mn((CH3COO)2) to the test tube
  3. Add 200μL of 40mM NH4CH3COO to the test tube
  4. Add 244μL of 0.55mg/mL protein to the test tube
  5. Add 352μL of 10mM NH4OH
    1. This should bring the pH up to 8.2. Check on this with a pH strip
  6. Bring the volume up to 2.5mL with water (Add 1504μL of water)
  7. Incubate at room temperature for 1 hour
  8. Add 200μL of 5mM Na2S
    1. When you add this add it drop wise (as best as you can) while vigorously shaking the test tube.
  9. Incubate at 37C for 5 days.

Data

In test tube one, extra water was added, bringing the total volume to 5.1mL.


Notes

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