840:153g:Projects/project4/2009/02/10

MoldBusters' Journal

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Tuesday 2/10 Josh and Casy Made glycerol stock from our transformed cultures stored in -80 degree freezer. Isolated plasmid DNA from transformed cultures and got them ready for running on the agarose gel on Thursday. Oggie, Derek, and Katy Boiled our bacteria in the thermocycler in an attempt to make a quick DNA extract. We used that DNA, as well as our old DNA from the previous extaction to clone the aprE gene. We will be running our sampels, as well as controls on an 1% agarose gel on Thursday.