840:153g:Projects/project16/2011/10/04
From OpenWetWare
Jump to navigationJump to search
Project name | Main project page Previous entry Next entry |
Gel Electrophoresis of PCR and Creating Lysis BufferToday, we ran our PCR products through a gel to determine if we successfully amplified our gene. Unfortunately, we failed to amplify our gene and we forgot to add the master mix to 2 of our 3 controls. There are many different things that could have made our PCR failed which include a failed DNA extraction, incorrect annealing temperatures, an incorrect master mix, etc. So we elected to try a different extraction protocol that we acquired from Dr. Spradling. In preparation for Thursday's lab, we created our lysis buffer.
|