Today in the lab, we conducted a gel electrophoresis on the PCR products from the previous lab. We used the gel to make sure our second segment of DNA, the "B" segment, would show a band at around 300 bp, and to test which of three annealing temperatures is the best for our "A" segment of DNA. The band for the A segment should be around 800 bp. The results showed a bright band in the lane for the B segment at around 300 bp and a bright band in two lanes designated to two different annealing temperatures for A segments at around 800 bp. We got the results we wanted, so next lab we are going to run another PCR reaction using just the A forward primer and B reverse primer to make the two segments into one segment at around 1100 bp and amplify it.
Written by: Megan Hughes