|Project name|| Main project page|
Previous entry Next entry
Tuesday: We digested our 9 remaining colonies yet to be screened for our (gene&double terminator)superpart and ran on a gel. results from this gel of the 9 remaining colonies resembled that from previous gels we ran. Base pair length ended up between 100-200bp so we believe there is a problem with the Ecor1 during the ligation process preventing our two parts break apart correctly in order to ligate them together.
We also religated our left over Gene & double terminator parts that we had stored. In preperation for another bacteria transformation.
Thursday: transformed bacteria with our newly religated gene & double terminator superpart. In preperation for a digestion and gel electrophoresis.