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Prepared LB agar and stored overnight in refrigerator on Tuesday. Thursday we autoclaved the agar, poured two control plates, added antibiotics. KAN to 700ml of agar in one flask. Added X Gal, IPPG, and Ampicilin (each 800ul) to the other agar flask. Poured plates, let sit, and stored. Thursday we also isolated our plasmid containing our gene and stored in freezer.