20.109: Spring 2007 schedule

MODULE	DAY	DATE	LECTURER	TA	LAB	note
		Tue Feb 6	NK		Orientation
1	1	Thur Feb 8	DE	SC	Start-up genome engineering	PDF of class discussion slides
1	2	Tue Feb 13	DE	SC	Agarose gel electrophoresis	PDF of class notes
1	3	Thur Feb 15	DE	SC	DNA ligation and bacterial transformation
		President's Day Feb 19				No lab Feb 20 or Feb 21
1	4	Thur Feb 22	DE	SC	Examine candidate clones	guest lecture from Harlan Breindel
1	5	Tue Feb 27	DE	SC	Western analysis	PDF of class discussion slides
1	6	Thur Mar 1	DE	SC	Probe western	first draft of your writing assignment due before lab, PDF of class slides
2	1	Tue Mar 6	AJ	YSN	Start-up signal measurement
2	2	Thur Mar 8	AJ	YSN	Measuring calcium in vitro
2	3	Tue Mar 13	AJ	YSN	Lipofection
2	4	Thur Mar 15	AJ	YSN	Measuring calcium in vivo
2	5	Tue Mar 20	AJ	YSN	Calcium signaling in vivo
2	6	Thur Mar 22	AJ	YSN	Student presentations
		Spring Break Mar 26-30				No lab all week
3	1	Tue Apr 3	NK	AT	Start-up expression engineering	PDF of M3D1 lecture slides
3	2	Thur Apr 5	NK	AT	Yeast transformation	PDF of M3D2 lecture slides
3	3	Tue Apr 10	NK	AT	Colony PCR	guest lecture: Susan Ruff and her slides are here
3	4	Thur Apr 12	NK	AT	Screen for phenotypes, isolate RNA	PDF of M3D4 lecture slides
		Patriot's Day April 16-17				No lab Apr 17 or Apr 18
3	5	Thur Apr 19	NK	AT	cDNA synthesis and microarray	PDF of M3D5 lecture slides
3	6	Tue Apr 24	NK	AT	Microarray data analysis	guest lecture: Rebecca Fry and her slides are here
4	1	Thur Apr 26	AB	CV	Screening library
4	2	Tue May 1	AB	CV	Optimizing panning	first draft of your writing assignment due Monday April 30th by 9AM
4	3	Thur May 3	AB	CV	Rescreening gold binders
4	4	Tue May 8	AB	CV	PCR of gold binding candidates	Mod 3 lab reports returned for revision
4	5	Thur May 10	AB	CV	Student presentations
4	6	Tue May 15	AB	CV	Analyze sequence data	Revised Mod 3 writing assignment due before lab no lecture today
		Thur May 17			Lecture only, no lab