20.109(S12): Spring 2012 schedule

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20.109(S12): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2012        Assignments       
RNA Engineering        Protein Engineering        Cell Engineering              

MODULE DAY DATE LECTURER TA LAB NOTES
Tue Feb 7 ANS JRR Orientation * Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
1 1 Thur Feb 9 JN JRR Amplify aptamer-encoding DNA * M1D1 Lecture
* M1D1 Pre-Lab
* Writing tutor talk
1 2 Tue Feb 14 JN JRR Purify aptamer-encoding DNA * M1D2 Lecture
* M1D2 Pre-Lab
* T/R WAC talk
* W/F WAC talk
1 3 Thur Feb 16 JN JRR Prepare RNA by IVT * M1D3 Lecture
* M1D3 Pre-Lab
* Oral presentation talk
President's Day Feb 20 No lab Feb 21 or Feb 22
1 4 Thur Feb 23 JN JRR Purify RNA and run affinity column * M1D4 Lecture
* M1D4 Pre-Lab
1 5 Tue Feb 28 JN JRR RNA to DNA by RT-PCR * M1D5 Lecture
* M1D5 Pre-Lab
1 6 Thur Mar 1 JN JRR Post-selection IVT and journal club * M1D6 Lecture
* M1D6 Pre-Lab
1 7 Tue Mar 6 JN JRR Aptamer binding assay * M1D7 Lecture
* M1D7 Pre-Lab
1 8 Thur Mar 8 JN JRR Journal club * M1D8 Lecture
3 1 Tue Mar 13 ANS HHH Start-up biomaterials engineering * M3D1 Lecture
* M3D1 Pre-Lab
Module 1 Draft Due
2 1 Thur Mar 15 AJ FF Start-up protein engineering * M2D1 Lecture
* M2D1 Pre-Lab
Module 1 Computational Assignment Due
2 2 Tue Mar 20 AJ FF Site-directed mutagenesis * M2D2 Lecture
* M2D2 Pre-Lab
2 3 Thur Mar 22 AJ FF Bacterial amplification of DNA * M2D3 Lecture
* M2D3 Pre-Lab
Spring Break Mar 26-30 No lab all week
2 4 Tue Apr 3 AJ FF Prepare expression system * M2D4 Lecture
* M2D4 Pre-Lab
2 5 Thur Apr 5 AJ FF Induce protein and evaluate DNA * M2D5 Lecture
* M2D5 Pre-Lab
Module 1 Final Report Due
2 6 Tue Apr 10 AJ FF Characterize protein expression * M2D6 Lecture (got through slide 15)
* M2D6 Pre-Lab
2 7 Thur Apr 12 AJ FF Assess protein function * M2D7 Lecture
* M2D7 Pre-Lab
Patriot's Day April 16-17 No lab Apr 17 or Apr 18
3 2 Thur Apr 19 ANS HHH Initiate cell culture * M3D2 Lecture
Tue Apr 24 ANS HHH No lab or lecture. Module 2 Draft due MONDAY April 23rd by 1 pm. (Changed to April 24th.)
3 3 Thur Apr 26 ANS HHH Testing cell viability * M3D3 Lecture
3 4 Tue May 1 ANS HHH Preparing cells for analysis * M3D4 Lecture
* M3D4 Pre-Lab
3 5 Thur May 3 ANS HHH Initiating transcript and protein assays * M3D5 Lecture
* M3D5 Pre-Lab
3 6 Tue May 8 ANS HHH Transcript-level analysis Module 2 Final Report Due
* M3D6 Lecture
* M3D6 Pre-Lab
3 7 Thur May 10 ANS HHH Protein-level and wrap-up analysis Module 3 Summary Due
3 8 Tue May 15 ANS HHH Student presentations
Thur May 17 No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
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