Beta-lactamase

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Colorimetric reporter for B-lac activity

Plasmid stabilization

  • Beta-lactamase gene bla or ampR is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and carbenicillin.

NITROCEFIN (GLAXO RESEARCH 87/312)
Code: SR0112
For the rapid chromogenic detection of beta-lactamase activity.
Oxiod Inc. page on Nitrocefin

References

  1. ANDERSON ES and DATTA N. RESISTANCE TO PENICILLINS AND ITS TRANSFER IN ENTEROBACTERIACEAE. Lancet 1965 Feb 20; 1(7382) 407-9. pmid:14238093. PubMed HubMed PubGet [Anderson-Lancet-1965]
  2. Datta N and Richmond MH. The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli. Biochem J 1966 Jan; 98(1) 204-9. pmid:5328167. PubMed HubMed PubGet [Datta-BiochemJ-1966]
  3. Evans J, Galindo E, Olarte J, and Falkow S. Beta-lactamase of R factors. J Bacteriol 1968 Oct; 96(4) 1441-2. pmid:4971890. PubMed HubMed PubGet [Evans-JBacteriol-1968]
  4. Joseph Sambrook, David W. Russell. Molecular cloning. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 2001. isbn:0-87969-577-3. [MolecularCloning]
  5. Gao W, Xing B, Tsien RY, and Rao J. Novel fluorogenic substrates for imaging beta-lactamase gene expression. J Am Chem Soc 2003 Sep 17; 125(37) 11146-7. doi:10.1021/ja036126o pmid:16220906. PubMed HubMed PubGet [Gao-JACS-2003]
All Medline abstracts: PubMed HubMed

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