ZEG reporter mouse: Difference between revisions
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"..We have developed a novel mouse line that acts as a Cre reporter. The mice, designated '''Z/EG (lacZ/EGFP)''', express lacZ throughout embryonic development and adult stages. Cre excision, however, removes the lacZ gene, which activates expression of the second reporter, enhanced green fluorescent protein. We have found that the double-reporter Z/EG line is able to '''indicate the occurrence of Cre excision from early embryonic to adult lineages'''..." | "..We have developed a novel mouse line that acts as a Cre reporter. The mice, designated '''Z/EG (lacZ/EGFP)''', express lacZ throughout embryonic development and adult stages. Cre excision, however, removes the lacZ gene, which activates expression of the second reporter, enhanced green fluorescent protein. We have found that the double-reporter Z/EG line is able to '''indicate the occurrence of Cre excision from early embryonic to adult lineages'''..." | ||
=== Transgene === | |||
start: 5' | |||
* promoter: pCAGGS | |||
* loxP site for Cre site-specific recombination | |||
* LacZ (bacterial β-galactosidase) | |||
* 3x pA signal | |||
* loxP site | |||
* e[[GFP]] | |||
* pA | |||
end: 3' | |||
Revision as of 06:57, 13 September 2010
Reporter mouse strain which can be switches by Cre recombination from LacZ to eGFP expression (therefore ZEG).
Description of the line
Original publication
- Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision.
- Genesis. 2000 Nov-Dec;28(3-4):147-55.
- Novak A, Guo C, Yang W, Nagy A, Lobe CG.
- Cancer Research Division, Sunnybrook and Women's College Health Science Centre, Toronto, Ontario, Canada.
- PMID 11105057
Excerpt from the abstract: "..We have developed a novel mouse line that acts as a Cre reporter. The mice, designated Z/EG (lacZ/EGFP), express lacZ throughout embryonic development and adult stages. Cre excision, however, removes the lacZ gene, which activates expression of the second reporter, enhanced green fluorescent protein. We have found that the double-reporter Z/EG line is able to indicate the occurrence of Cre excision from early embryonic to adult lineages..."
Transgene
start: 5'
- promoter: pCAGGS
- loxP site for Cre site-specific recombination
- LacZ (bacterial β-galactosidase)
- 3x pA signal
- loxP site
- eGFP
- pA
end: 3'
