Yu:TEV Cleavage Buffer

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Current revision (13:58, 24 December 2009) (view source)
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|+'''Solution recipe for 1L TEV Cleavage Buffer.'''
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|+'''Solution recipe for 500 mL TEV Cleavage Buffer.'''
|width="66%" valign="top"|1. Measure 100 mL millipore H<sub>2</sub>O into a 500mL beaker
|width="66%" valign="top"|1. Measure 100 mL millipore H<sub>2</sub>O into a 500mL beaker
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|width="33%" valign="top"|
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|5. Bring volume to 500L with millipore H<sub>2</sub>O in a graduated cylinder
|5. Bring volume to 500L with millipore H<sub>2</sub>O in a graduated cylinder
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|Volume to 1L
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|6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB
|6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB

Current revision

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Solution recipe for 500 mL TEV Cleavage Buffer.
1. Measure 100 mL millipore H2O into a 500mL beaker
2. Add 12.5 mL of 1M TRIS-HCl, pH 8.0 25 mM TRIS-HCl
3. Add 15 mL of 5M NaCl 150 mM NaCl
4. Add 500 μL of 0.5M EDTA 0.5 mM EDTA
5. Add 5 mL 10% NP-40 0.1% NP-40
5. Bring volume to 500L with millipore H2O in a graduated cylinder
6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB 1 mM DTT
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