Yu:TBS: Difference between revisions
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|+'''Solution recipe for 1L 10x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.''' | |+'''Solution recipe for 1L 10x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.''' | ||
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker. | |width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker. | ||
|width="100px" valign="top"|<u> | |width="100px" valign="top"|<u>10x</u> | ||
|- | |- | ||
|2. Weigh and add 87.7g NaCl | |2. Weigh and add 87.7g NaCl |
Latest revision as of 15:07, 20 September 2011
1. Measure 700mL millipore water into a 2L plastic beaker. | 10x |
2. Weigh and add 87.7g NaCl | 1.5M NaCl |
3. Add 100mL 1M TRIS-HCl pH 7.5 | 100mM TRIS pH 7.5 |
4. Stir until dissolved | |
5. Bring volume to 1L with millipore water in a graduated cylinder | |
6. Dilute to 1x (1:10) for use |
1. Make 1L of 1x TBS by diluting 10x stock with millipore H2O | |
2. Add 2.5mL TWEEN-20 | 0.25% TWEEN |
3. Add 10mL Triton-X | 1% Triton-X |
Vary detergents by intended application |