Yu:TBS

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|+'''Solution recipe for 1L 10x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.'''
|+'''Solution recipe for 1L 10x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.'''
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
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|width="100px" valign="top"|<u>5x</u>
+
|width="100px" valign="top"|<u>10x</u>
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|-
|2. Weigh and add 87.7g NaCl
|2. Weigh and add 87.7g NaCl

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Solution recipe for 1L 10x Tris-Buffered Saline. Dilute to 1x for use.
1. Measure 700mL millipore water into a 2L plastic beaker. 10x
2. Weigh and add 87.7g NaCl 1.5M NaCl
3. Add 100mL 1M TRIS-HCl pH 7.5 100mM TRIS pH 7.5
4. Stir until dissolved
5. Bring volume to 1L with millipore water in a graduated cylinder
6. Dilute to 1x (1:10) for use
Solution recipe for 1L Tris-Buffered Saline / Tween-Triton
1. Make 1L of 1x TBS by diluting 10x stock with millipore H2O
2. Add 2.5mL TWEEN-20 0.25% TWEEN
3. Add 10mL Triton-X 1% Triton-X
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