Yu:TBS

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (sp)
m
Line 2: Line 2:
{| border="0" cellpadding="10"
{| border="0" cellpadding="10"
-
|+'''Solution recipe for 1L 5x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.'''
+
|+'''Solution recipe for 1L 10x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.'''
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
|width="100px" valign="top"|<u>5x</u>
|width="100px" valign="top"|<u>5x</u>
|-
|-
-
|2. Weigh and add 43.83g NaCl, or add 150mL 5M NaCl
+
|2. Weigh and add 87.7g NaCl
-
|750mM NaCl
+
|1.5M NaCl
|-
|-
-
|3. Add 50mL 1M TRIS-HCl pH 7.5
+
|3. Add 100mL 1M TRIS-HCl pH 7.5
-
|50mM TRIS pH 7.5
+
|100mM TRIS pH 7.5
|-
|-
|4. Stir until dissolved
|4. Stir until dissolved
Line 18: Line 18:
|
|
|-
|-
-
|6. Dilute from 5x to 1x (1:5) for use
+
|6. Dilute to 1x (1:10) for use
|}
|}
{| border="0" cellpadding="10"
{| border="0" cellpadding="10"
-
|+'''Solution recipe for 1L 5x <span id="TBS-TT">Tris-Buffered Saline / Tween-Triton</span>. Dilute to 1x for use.'''
+
|+'''Solution recipe for 1L <span id="TBS-TT">Tris-Buffered Saline / Tween-Triton</span>'''
-
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
+
|width="600px" valign="top"|1. Make 1L of 1x TBS by diluting 10x stock with millipore H<sub>2</sub>O
-
|width="100px" valign="top"|<u>5x</u>
+
|
|-
|-
-
|2. Weigh and add 146.1g NaCl, or add 500mL 5M NaCl
+
|2. Add 2.5mL TWEEN-20
-
|2.5M NaCl
+
|0.25% TWEEN
|-
|-
-
|3. Add 100mL 1M TRIS-HCl pH 7.5
+
|3. Add 10mL Triton-X
-
|100mM TRIS pH 7.5
+
|1% Triton-X
-
|-
+
-
|4. Add 2.5mL Tween-20
+
-
|0.25% Tween-20
+
-
|-
+
-
|5. Add 10mL Triton-X100
+
-
|1% Triton-X100
+
-
|-
+
-
|6. Stir until dissolved
+
-
|
+
-
|-
+
-
|7. Bring volume to 1L with millipore water in a graduated cylinder
+
-
|
+
|-
|-
-
|8. Dilute from 5x to 1x (1:5) for use
+
|Vary detergents by intended application
|}
|}

Revision as of 18:07, 20 September 2011

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database       Image:UBLogo.jpg


Solution recipe for 1L 10x Tris-Buffered Saline. Dilute to 1x for use.
1. Measure 700mL millipore water into a 2L plastic beaker. 5x
2. Weigh and add 87.7g NaCl 1.5M NaCl
3. Add 100mL 1M TRIS-HCl pH 7.5 100mM TRIS pH 7.5
4. Stir until dissolved
5. Bring volume to 1L with millipore water in a graduated cylinder
6. Dilute to 1x (1:10) for use
Solution recipe for 1L Tris-Buffered Saline / Tween-Triton
1. Make 1L of 1x TBS by diluting 10x stock with millipore H2O
2. Add 2.5mL TWEEN-20 0.25% TWEEN
3. Add 10mL Triton-X 1% Triton-X
Vary detergents by intended application
Personal tools