Yu:TBS

(Difference between revisions)

Revision as of 19:57, 9 May 2010

Home Procedures | Lab Members | Publications | Research Interests | Internal Database

**Solution recipe for 1L 5x Tris-Buffered Saline. Dilute to 1x for use.**
1. Measure 700mL millipore water into a 2L plastic beaker.	5x
2. Weigh and add 43.83g NaCl, or add 150mL 5M NaCl	750mM NaCl
3. Add 50mL 1M TRIS-HCl pH 7.5	50mM TRIS pH 7.5
4. Stir until dissolved
5. Bring volume to 1L with millipore water in a graduated cylinder
6. Dilute from 5x to 1x (1:5) for use