Yu:TBS: Difference between revisions
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{| border="0" cellpadding="10" | {| border="0" cellpadding="10" | ||
|+'''Solution recipe for 1L | |+'''Solution recipe for 1L 10x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.''' | ||
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker. | |width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker. | ||
|width="100px" valign="top"|<u> | |width="100px" valign="top"|<u>10x</u> | ||
|- | |- | ||
|2. Weigh and add | |2. Weigh and add 87.7g NaCl | ||
| | |1.5M NaCl | ||
|- | |- | ||
|3. Add | |3. Add 100mL 1M TRIS-HCl pH 7.5 | ||
| | |100mM TRIS pH 7.5 | ||
|- | |- | ||
|4. Stir until dissolved | |4. Stir until dissolved | ||
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| | | | ||
|- | |- | ||
|6. Dilute | |6. Dilute to 1x (1:10) for use | ||
|} | |} | ||
{| border="0" cellpadding="10" | {| border="0" cellpadding="10" | ||
|+'''Solution recipe for 1L | |+'''Solution recipe for 1L <span id="TBS-TT">Tris-Buffered Saline / Tween-Triton</span>''' | ||
|width="600px" valign="top"|1. | |width="600px" valign="top"|1. Make 1L of 1x TBS by diluting 10x stock with millipore H<sub>2</sub>O | ||
| | |||
| | |||
|- | |- | ||
| | |2. Add 2.5mL TWEEN-20 | ||
|0.25% | |0.25% TWEEN | ||
|- | |- | ||
| | |3. Add 10mL Triton-X | ||
|1% Triton-X | |1% Triton-X | ||
|- | |- | ||
| | |Vary detergents by intended application | ||
|} | |} |
Latest revision as of 15:07, 20 September 2011
1. Measure 700mL millipore water into a 2L plastic beaker. | 10x |
2. Weigh and add 87.7g NaCl | 1.5M NaCl |
3. Add 100mL 1M TRIS-HCl pH 7.5 | 100mM TRIS pH 7.5 |
4. Stir until dissolved | |
5. Bring volume to 1L with millipore water in a graduated cylinder | |
6. Dilute to 1x (1:10) for use |
1. Make 1L of 1x TBS by diluting 10x stock with millipore H2O | |
2. Add 2.5mL TWEEN-20 | 0.25% TWEEN |
3. Add 10mL Triton-X | 1% Triton-X |
Vary detergents by intended application |