Yu:TAE: Difference between revisions
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Michaelcyu (talk | contribs) (50xTAE) |
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Revision as of 10:53, 7 June 2009
1. Measure 600mL H2O into a 2L plastic beaker. | 600mL H2O |
2. Weigh 242g Tris base and add to mixture | 242g Tris base |
3. Measure 57.1mL acetic acid (glacial) and add to mixture | 57.1mL acetic acid (glacial) |
4. Measure 100mL .5M EDTA and add to mixture | 100mL .5M EDTA |
5. Stir until completely dissolved | |
6. Bring volume to 1L in a graduated cylinder | Volume to 1L |
7. Dilute from 50x to 1x (1:50) for use. |