Yu:SOC

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|+'''Solution recipe for SOC'''.
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|+'''Solution recipe for 100mL SOC'''
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|width="600px" valign="top"|1. Measure 50mL millipore water into a 200mL beaker.
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|width="100px" valign="top"|
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|-
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|For 100ml
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|2. Weigh 2g Tryptone and add it to the mixture
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|-
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|2% (w/v) Tryptone
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|1. 2g tryptone (2%w/v)
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|-
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|2. 0.5 g yeast extract (0.5%w/v)
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|-
|-
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|3. 330μL 3M NaCl(10mM)
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|5. Weigh 0.5g yeast extract and add it to the mixture
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|0.5% (w/v) Yeast Extract
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|-
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|4. 83μL 3M KCl (205mM)
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|4. Pipette 200μL of 5M NaCl into the mixture
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|10mM NaCl
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|-
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|5. 1 ml 0.1 M MgCl<sub>2</sub>
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|5. Pipette 83μL of 3M KCl into the mixture
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|2.5mM KCl
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|-
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|6.0.245g MgSO<sub>4</sub> (10mM)
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|6. Pipette 100μL of 1M MgCl<sub>2</sub> into the mixture
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|10mM MgCl<sub>2</sub>
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|-
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|7. 0.36g dextrose(20mM)
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|7. Weigh 0.245g MgSO<sub>4</sub> and add it to the mixture
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|10mM MgSO<sub>4</sub>
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|-
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|8. Weigh 0.36g dextrose and add it to the mixture
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|20mM dextrose
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|-
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|9. Bring to 100mL with millipore water
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|
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[[Category:Yu Solutions]]
[[Category:Yu Solutions]]

Revision as of 18:11, 22 February 2011

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Solution recipe for 100mL SOC
1. Measure 50mL millipore water into a 200mL beaker.
2. Weigh 2g Tryptone and add it to the mixture 2% (w/v) Tryptone
5. Weigh 0.5g yeast extract and add it to the mixture 0.5% (w/v) Yeast Extract
4. Pipette 200μL of 5M NaCl into the mixture 10mM NaCl
5. Pipette 83μL of 3M KCl into the mixture 2.5mM KCl
6. Pipette 100μL of 1M MgCl2 into the mixture 10mM MgCl2
7. Weigh 0.245g MgSO4 and add it to the mixture 10mM MgSO4
8. Weigh 0.36g dextrose and add it to the mixture 20mM dextrose
9. Bring to 100mL with millipore water
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