Yu:SOC

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Revision as of 19:11, 22 February 2011

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**Solution recipe for 100mL SOC**
1. Measure 50mL millipore water into a 200mL beaker.
2. Weigh 2g Tryptone and add it to the mixture	2% (w/v) Tryptone
5. Weigh 0.5g yeast extract and add it to the mixture	0.5% (w/v) Yeast Extract
4. Pipette 200μL of 5M NaCl into the mixture	10mM NaCl
5. Pipette 83μL of 3M KCl into the mixture	2.5mM KCl
6. Pipette 100μL of 1M MgCl₂ into the mixture	10mM MgCl₂
7. Weigh 0.245g MgSO₄ and add it to the mixture	10mM MgSO₄
8. Weigh 0.36g dextrose and add it to the mixture	20mM dextrose
9. Bring to 100mL with millipore water

@@ Line 1: / Line 1: @@
 {{Template:Yu}}
-{| border="0" cellpadding="10"
+{|border="0" cellpadding="10"
-|+'''Solution recipe for  SOC'''.
+|+'''Solution recipe for 100mL SOC'''
+|width="600px" valign="top"|1. Measure 50mL millipore water into a 200mL beaker.
+|width="100px" valign="top"|
 |-
-|For 100ml
+|2. Weigh 2g Tryptone and add it to the mixture
-|-
+|2% (w/v) Tryptone
-|1. 2g tryptone (2%w/v)
-|-
-|2. 0.5 g yeast extract (0.5%w/v)
 |-
-|3. 330μL 3M NaCl(10mM)
+|5. Weigh 0.5g yeast extract and add it to the mixture
+|0.5% (w/v) Yeast Extract
 |-
-|4. 83μL 3M KCl (205mM)
+|4. Pipette 200μL of 5M NaCl into the mixture
+|10mM NaCl
 |-
-|5. 1 ml 0.1 M MgCl<sub>2</sub>
+|5. Pipette 83μL of 3M KCl into the mixture
+|2.5mM KCl
 |-
-|6.0.245g MgSO<sub>4</sub> (10mM)
+|6. Pipette 100μL of 1M MgCl<sub>2</sub> into the mixture
+|10mM MgCl<sub>2</sub>
 |-
-|7. 0.36g dextrose(20mM)
+|7. Weigh 0.245g MgSO<sub>4</sub> and add it to the mixture
+|10mM MgSO<sub>4</sub>
+|-
+|8. Weigh 0.36g dextrose and add it to the mixture
+|20mM dextrose
+|-
+|9. Bring to 100mL with millipore water
+|
 |}
 [[Category:Yu Solutions]]