Yu:RIPA-Buffer

Solution recipe for RIPA Buffer.

For 250ml:

1. 50 mM Tris pH 8.0( 12.5ml of 1M Stock)

2. 150 mM NaCl(7.5 l of 5M Stock)

3. 1% NP-40(205ml of stock

4. 0.5% of Na-DOC(12.5 ml of 10% w/v stock)

5. 0.1% SDS(2.5ml of 10% stock)

6. 212.5 ml water