Yu:Procedures

Solutions YEPD Dropout Media Lysis Buffer SDS-PAGE Buffer Transfer Buffer for Western Blot PBS PBST PBSMT 50x TAE 10x TBE LiOAc/TE IPP150 IPP300 NP-40 Buffer TEV Cleavage Buffer Calmodulin-Binding Buffer TES

Western Blot Lysing Cells SDS-PAGE Gel Western Transfer Immunoblotting Developing Stripping Transformation Yeast LiOAc Transformation E. coli Transformation Quick plasmid mini prep Southern Blot Solutions Southern Blotting CoIP TAP CoIP PCR Homemade Taq ChIP PCR (qPCR) Spheroplast Preperations Solutions Spheroplast preperation Nuclei Isolation ChIP 8% TBE polyacrylamide gel Northern Blot RNA extraction Agarose-Formaldehyde gels High-resolution gels Northern Solutions Oligo End Labeling Chromatin mapping MNase digestion Probe Design Cloning TOPO TA Manual