Yu:Procedures

Solutions YEPD Dropout Media Lysis Buffer SDS-PAGE Buffer Transfer Buffer for Western Blot PBS PBST PBSMT 50x TAE 10x TBE LiOAc/TE IPP150 IPP300 NP-40 Buffer TEV Cleavage Buffer Calmodulin-Binding Buffer TES

Western Blot Lysing Cells SDS-PAGE Gel Western Transfer Immunoblotting Developing Transformation Yeast LiOAc Transformation E. coli Transformation Southern Blot Solutions Southern Blotting CoIP PCR Homemade Taq Spheroplast Preperations Solutions Spheroplast preperation Nuclei Isolation ChIP 8% TBE polyacrylamide gel Northern Blot RNA extraction Agarose-Formaldehyde gels High-resolution gels Northern Solutions Oligo End Labeling Chromatin mapping MNase digestion Probe Design