Yu:Procedures

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (competent cells)
m (link)
(4 intermediate revisions not shown.)
Line 5: Line 5:
*[[Yu:YEPD|YEPD]]
*[[Yu:YEPD|YEPD]]
 +
*[[Yu:SOC|SOC]]
*[[Yu:Dropout Media|Dropout Media]]
*[[Yu:Dropout Media|Dropout Media]]
*[[Yu:Lysis#buffer|Lysis Buffer]]
*[[Yu:Lysis#buffer|Lysis Buffer]]
Line 23: Line 24:
*[[Yu:CBB|Calmodulin-Binding Buffer]]
*[[Yu:CBB|Calmodulin-Binding Buffer]]
*[[Yu:TES|TES]]
*[[Yu:TES|TES]]
 +
*[[Yu:Super_SPO|Super SPO Media]]
 +
*[[Yu:P Solution|P Solution]]
|width="70%" valign="top" align="left"|  
|width="70%" valign="top" align="left"|  
Line 42: Line 45:
*[[Yu:e_coli_transform|''E. coli'' Transformation]]
*[[Yu:e_coli_transform|''E. coli'' Transformation]]
*[[Yu:Competent_Cells|''E. coli'' Normal Competent Cells]]
*[[Yu:Competent_Cells|''E. coli'' Normal Competent Cells]]
-
*[[Yu:quick_miniprep|Quick plasmid mini prep]]
+
*[[Yu:quick_miniprep|Quick E. coli plasmid mini prep]]
 +
*[[Yu:Yeast_miniprep|Yeast Plasmid Miniprep]]
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Southern Blot</font></div>
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Southern Blot</font></div>
Line 82: Line 86:
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Cloning</font></div>
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Cloning</font></div>
*[http://tools.invitrogen.com/content/sfs/manuals/topota_man.pdf TOPO TA Manual]
*[http://tools.invitrogen.com/content/sfs/manuals/topota_man.pdf TOPO TA Manual]
 +
*[[Yu:Genomic_prep|Rapid Genomic Prep]]
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Misc</font></div>
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Misc</font></div>
*[[Yu:Dialysis|Dialysis]]
*[[Yu:Dialysis|Dialysis]]

Revision as of 19:59, 1 December 2012

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database       Image:UBLogo.jpg


Solutions


Transformation
Southern Blot
CoIP
PCR
Spheroplast Preperations
ChIP
Northern Blot
Chromatin mapping
Cloning
Misc
Personal tools