# Yu:Procedures

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 Revision as of 14:57, 13 September 2009 (view source)m (added homemade taq link)← Previous diff Revision as of 19:59, 1 December 2012 (view source)m (link)Next diff → (13 intermediate revisions not shown.) Line 5: Line 5: *[[Yu:YEPD|YEPD]] *[[Yu:YEPD|YEPD]] + *[[Yu:SOC|SOC]] *[[Yu:Dropout Media|Dropout Media]] *[[Yu:Dropout Media|Dropout Media]] *[[Yu:Lysis#buffer|Lysis Buffer]] *[[Yu:Lysis#buffer|Lysis Buffer]] Line 12: Line 13: **[[Yu:PBS#PBST|PBST]] **[[Yu:PBS#PBST|PBST]] **[[Yu:PBS#PBSMT|PBSMT]] **[[Yu:PBS#PBSMT|PBSMT]] - *[[Yu:TAE|TAE]] + *[[Yu:TBS|TBS]] - *[[Yu:TBE|TBE]] + **[[Yu:TBS#TBS-TT|TBS/TT]] + *[[Yu:TAE|50x TAE]] + *[[Yu:TBE|10x TBE]] *[[Yu:LiOAc|LiOAc/TE]] *[[Yu:LiOAc|LiOAc/TE]] *[[Yu:IPP|IPP150]] *[[Yu:IPP|IPP150]] Line 21: Line 24: *[[Yu:CBB|Calmodulin-Binding Buffer]] *[[Yu:CBB|Calmodulin-Binding Buffer]] *[[Yu:TES|TES]] *[[Yu:TES|TES]] + *[[Yu:Super_SPO|Super SPO Media]] + *[[Yu:P Solution|P Solution]] |width="70%" valign="top" align="left"| |width="70%" valign="top" align="left"| Line 32: Line 37: *[[Yu:Western#Immunoblotting|Immunoblotting]] *[[Yu:Western#Immunoblotting|Immunoblotting]] *[[Yu:Western#Developing|Developing]] *[[Yu:Western#Developing|Developing]] + *[[Yu:Western#Stripping|Stripping]] Line 38: Line 44: *[[Yu:Transformation|Yeast LiOAc Transformation]] *[[Yu:Transformation|Yeast LiOAc Transformation]] *[[Yu:e_coli_transform|''E. coli'' Transformation]] *[[Yu:e_coli_transform|''E. coli'' Transformation]] + *[[Yu:Competent_Cells|''E. coli'' Normal Competent Cells]] + *[[Yu:quick_miniprep|Quick E. coli plasmid mini prep]] + *[[Yu:Yeast_miniprep|Yeast Plasmid Miniprep]] |width="33%" valign="top" align="left"|
Southern Blot
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Southern Blot
Line 45: Line 54: |width="33%" valign="top" align="left"|
CoIP
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CoIP
+ *[[Yu:CoIP|TAP CoIP]] |width="33%" valign="top" align="left"|
PCR
|width="33%" valign="top" align="left"|
PCR
*[[Yu:homemade_taq|Homemade Taq]] *[[Yu:homemade_taq|Homemade Taq]] + *[[Yu:ChIP_PCR|ChIP PCR (qPCR)]] |width="33%" valign="top" align="left"|
Spheroplast Preperations
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Spheroplast Preperations
Line 73: Line 84: *[[Yu:probe_design|Probe Design]] *[[Yu:probe_design|Probe Design]] |- |- + |width="33%" valign="top" align="left"|
Cloning
+ *[http://tools.invitrogen.com/content/sfs/manuals/topota_man.pdf TOPO TA Manual] + *[[Yu:Genomic_prep|Rapid Genomic Prep]] + |width="33%" valign="top" align="left"|
Misc
+ *[[Yu:Dialysis|Dialysis]] |} |} |- |- |} |}

## Revision as of 19:59, 1 December 2012

Solutions
 Western Blot Transformation Southern Blot CoIP TAP CoIP PCR Spheroplast Preperations ChIP 8% TBE polyacrylamide gel Northern Blot Chromatin mapping Cloning Misc Dialysis