Yu:Procedures

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Line 5: Line 5:
*[[Yu:YEPD|YEPD]]
*[[Yu:YEPD|YEPD]]
 +
*[[Yu:SOC|SOC]]
*[[Yu:Dropout Media|Dropout Media]]
*[[Yu:Dropout Media|Dropout Media]]
*[[Yu:Lysis#buffer|Lysis Buffer]]
*[[Yu:Lysis#buffer|Lysis Buffer]]
Line 12: Line 13:
**[[Yu:PBS#PBST|PBST]]
**[[Yu:PBS#PBST|PBST]]
**[[Yu:PBS#PBSMT|PBSMT]]
**[[Yu:PBS#PBSMT|PBSMT]]
-
*[[Yu:TAE|TAE]]
+
*[[Yu:TBS|TBS]]
-
*[[Yu:TBE|TBE]]
+
**[[Yu:TBS#TBS-TT|TBS/TT]]
 +
*[[Yu:TAE|50x TAE]]
 +
*[[Yu:TBE|10x TBE]]
*[[Yu:LiOAc|LiOAc/TE]]
*[[Yu:LiOAc|LiOAc/TE]]
*[[Yu:IPP|IPP150]]
*[[Yu:IPP|IPP150]]
Line 21: Line 24:
*[[Yu:CBB|Calmodulin-Binding Buffer]]
*[[Yu:CBB|Calmodulin-Binding Buffer]]
*[[Yu:TES|TES]]
*[[Yu:TES|TES]]
 +
*[[Yu:Super_SPO|Super SPO Media]]
 +
*[[Yu:P Solution|P Solution]]
|width="70%" valign="top" align="left"|  
|width="70%" valign="top" align="left"|  
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*[[Yu:Western#Immunoblotting|Immunoblotting]]
*[[Yu:Western#Immunoblotting|Immunoblotting]]
*[[Yu:Western#Developing|Developing]]
*[[Yu:Western#Developing|Developing]]
 +
*[[Yu:Western#Stripping|Stripping]]
Line 38: Line 44:
*[[Yu:Transformation|Yeast LiOAc Transformation]]
*[[Yu:Transformation|Yeast LiOAc Transformation]]
*[[Yu:e_coli_transform|''E. coli'' Transformation]]
*[[Yu:e_coli_transform|''E. coli'' Transformation]]
 +
*[[Yu:Competent_Cells|''E. coli'' Normal Competent Cells]]
 +
*[[Yu:quick_miniprep|Quick E. coli plasmid mini prep]]
 +
*[[Yu:Yeast_miniprep|Yeast Plasmid Miniprep]]
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Southern Blot</font></div>
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Southern Blot</font></div>
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|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">CoIP</font></div>
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">CoIP</font></div>
 +
*[[Yu:CoIP|TAP CoIP]]
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">PCR</font></div>
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">PCR</font></div>
*[[Yu:homemade_taq|Homemade Taq]]
*[[Yu:homemade_taq|Homemade Taq]]
 +
*[[Yu:ChIP_PCR|ChIP PCR (qPCR)]]
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Spheroplast Preperations</font></div>
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Spheroplast Preperations</font></div>
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*[[Yu:probe_design|Probe Design]]
*[[Yu:probe_design|Probe Design]]
|-
|-
 +
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Cloning</font></div>
 +
*[http://tools.invitrogen.com/content/sfs/manuals/topota_man.pdf TOPO TA Manual]
 +
*[[Yu:Genomic_prep|Rapid Genomic Prep]]
 +
|width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Misc</font></div>
 +
*[[Yu:Dialysis|Dialysis]]
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Revision as of 18:59, 1 December 2012

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Transformation
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