Yu:PBS

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m (fixed mMs)
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|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''
|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
-
|width="100px" valign="top"|700mL H<sub>2</sub>O
+
|width="100px" valign="top"|<u>10x</u>
|-
|-
|2. Weigh 80g NaCl and add it to the mixture
|2. Weigh 80g NaCl and add it to the mixture
-
|80g NaCl
+
|1.4M NaCl
|-
|-
|5. Weigh 2g KCl and add it to the mixture
|5. Weigh 2g KCl and add it to the mixture
-
|2g KCl
+
|30mM KCl
|-
|-
|4. Weigh 14.4g Na<sub>2</sub>HPO<sub>4</sub> and add it to the mixture
|4. Weigh 14.4g Na<sub>2</sub>HPO<sub>4</sub> and add it to the mixture
-
|14.4g Na<sub>2</sub>HPO<sub>4</sub>
+
|100mM Na<sub>2</sub>HPO<sub>4</sub>
|-
|-
|5. Weigh 2.4g KH<sub>2</sub>PO<sub>4</sub> and add it to the mixture
|5. Weigh 2.4g KH<sub>2</sub>PO<sub>4</sub> and add it to the mixture
-
|2.4g KH<sub>2</sub>PO<sub>4</sub>
+
|20mM KH<sub>2</sub>PO<sub>4</sub>
|-
|-
|6. Stir until dissolved
|6. Stir until dissolved
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|-
|-
|7. Bring volume to 1L with millipore water in a graduated cylinder
|7. Bring volume to 1L with millipore water in a graduated cylinder
-
|Volume to 1L
+
|
|-
|-
|8. Dilute from 10x to 1x (1:10) for use
|8. Dilute from 10x to 1x (1:10) for use
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|-
|-
|2. Pipette 2.5mL [[Tween|TWEEN 20]] into the bottle
|2. Pipette 2.5mL [[Tween|TWEEN 20]] into the bottle
-
|2.5mL [[Tween|TWEEN 20]]
+
|0.25% [[Tween|TWEEN 20]]
|-
|-
|3. Stir until mixed
|3. Stir until mixed
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|width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]]
|width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]]
|-
|-
-
|2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM
+
|2. Add 3mL 1M KCl
-
|3mL 1M KCl  
+
|3mM KCl  
|-
|-
-
|3. Pipette 2.5mL 1M MgCl<sub>2</sub> into the bottle, to bring MgCl<sub>2</sub> concentration to 2.5mM
+
|3. Add 2.5mL 1M MgCl<sub>2</sub>
-
|2.5mL 1M MgCl<sub>2</sub>
+
|2.5mM MgCl<sub>2</sub>
|-
|-
-
|4. Pipette 5mL [[Triton X-100]] into the bottle, to bring Triton X-100 concentration to .5%
+
|4. Add 5mL [[Triton X-100]]
-
|5mL [[Triton X-100]]
+
|0.5% [[Triton X-100]]
 +
|-
 +
|5. Stir until mixed
 +
|
|}
|}
[[Category:Yu Solutions]]
[[Category:Yu Solutions]]

Revision as of 19:53, 9 May 2010

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Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.
1. Measure 700mL millipore water into a 2L plastic beaker. 10x
2. Weigh 80g NaCl and add it to the mixture 1.4M NaCl
5. Weigh 2g KCl and add it to the mixture 30mM KCl
4. Weigh 14.4g Na2HPO4 and add it to the mixture 100mM Na2HPO4
5. Weigh 2.4g KH2PO4 and add it to the mixture 20mM KH2PO4
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder
8. Dilute from 10x to 1x (1:10) for use


Solution recipe for 1L 1x PBST.
1. Measure 1L 1x PBS into a 1L bottle. 1L 1x PBS
2. Pipette 2.5mL TWEEN 20 into the bottle 0.25% TWEEN 20
3. Stir until mixed


Solution recipe for 1L 1x PBSMT.
1. Measure 1L 1x PBS into a 1L bottle. 1L 1x PBS
2. Add 3mL 1M KCl 3mM KCl
3. Add 2.5mL 1M MgCl2 2.5mM MgCl2
4. Add 5mL Triton X-100 0.5% Triton X-100
5. Stir until mixed
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