Yu:PBS

Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.

1. Measure 700mL millipore water into a 2L plastic beaker.	700mL H2O
2. Weigh 80g NaCl and add it to the mixture	80g NaCl
5. Weigh 2g KCl and add it to the mixture	2g KCl
4. Weigh 14.4g Na2HPO4 and add it to the mixture	14.4g Na2HPO4
5. Weigh 2.4g KH2PO4 and add it to the mixture	2.4g KH2PO4
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder	Volume to 1L
8. Dilute from 10x to 1x (1:10) for use

Solution recipe for 1L 1x PBST.

1. Measure 1L 1x PBS into a 1L bottle.	1L 1x PBS
2. Pipette 2.5mL TWEEN 20 into the bottle	2.5mL TWEEN 20
3. Stir until mixed

Solution recipe for 1L 1x PBSMT.

1. Measure 1L 1x PBS into a 1L bottle.	1L 1x PBS
2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM	3mL 1M KCl
3. Pipette 2.5mL 1M MgCl2 into the bottle, to bring MgCl2 concentration to 2.5mM	2.5mL 1M MgCl2
4. Pipette 5mL Triton X-100 into the bottle, to bring Triton X-100 concentration to .5%	5mL Triton X-100

Solution recipe for PBS KMT .

1. 20mL PBS (10X)

2. 600 μL 1M KCl=3mM Final concentration

3. 500μL 1M MgCl2=2.5mM final concentration

4. 10mL 10%-Triton X-100

5. Fill to 200mL with Milipore H2O