Yu:PBS: Difference between revisions

Revision as of 10:19, 7 June 2009

Home Procedures | Lab Members | Publications | Research Interests | Internal Database

**Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.**
1. Measure 700mL millipore water into a 2L plastic beaker.	700mL H₂O
2. Weigh 80g NaCl and add it to the mixture	80g NaCl
5. Weigh 2g KCl and add it to the mixture	2g KCl
4. Weigh 14.4g Na₂HPO₄ and add it to the mixture	14.4g Na₂HPO₄
5. Weigh 2.4g KH₂PO₄ and add it to the mixture	2.4g KH₂PO₄
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder	Volume to 1L
8. Dilute from 10x to 1x (1:10) for use

**Solution recipe for 1L 1x PBST**.
1. Measure 1L 1x PBS into a 1L bottle.	1L 1x PBS
2. Pipette 2.5mL TWEEN 20 into the bottle	2.5mL TWEEN 20
3. Stir until mixed

@@ Line 2: / Line 2: @@
 {| border="0" cellpadding="10"
-|+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.'''
+|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''
-|width="80%" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
+|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
-|width="20%" valign="top"|700mL H<sub>2</sub>O
+|width="100px" valign="top"|700mL H<sub>2</sub>O
 |-
 |2. Weigh 80g NaCl and add it to the mixture
 |80g NaCl
 |-
-|3. Weigh 2g KCl and add it to the mixture
+|5. Weigh 2g KCl and add it to the mixture
 |2g KCl
 |-
@@ Line 25: / Line 25: @@
 |-
 |8. Dilute from 10x to 1x (1:10) for use
+|}
+{| border="0" cellpadding="10"
+|+'''Solution recipe for 1L 1x <span id="PBST">PBST</span>'''.
+|width="600px" valign="top"|1. Measure 1L 1x PBS into a 1L bottle.
+|width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]]
+|-
+|2. Pipette 2.5mL TWEEN 20 into the bottle
+|2.5mL TWEEN 20
+|-
+|3. Stir until mixed
+|
 |}