Yu:EDTA: Difference between revisions

Revision as of 10:09, 18 September 2009

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**Solution recipe for 0.5M EDTA**.
1. Add 186.1 g of Sodium ethylenediaminetetraacetate.H₂O to 800ml of H₂O.
2. Stir vigorously on a magnetic stirrer.
3. Adjust he PpH to 8.0 with NaOH (~20g of NaOH pellets)

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 {| border="0" cellpadding="10"
-|+'''Solution recipe for 1M CaCl<sub>2</sub>'''.
+|+'''Solution recipe for 0.5M EDTA'''.
-|1. Dissolve 54g  of CaCl<sub>2</sub>.6H<sub>2</sub>O in 200ml of pure H<sub>2</sub>O(Milli-Q or equivalent).
+|1. Add 186.1 g of Sodium ethylenediaminetetraacetate.H<sub>2</sub>O to 800ml of H<sub>2</sub>O.
 |-
-|2. Sterilize the solution by passage through a 0.22-micron filter.
+|2. Stir vigorously on a magnetic stirrer.
+|-
+|3. Adjust he PpH to 8.0 with NaOH (~20g of NaOH pellets)
 |-
-|3. Store in 1-ml aliquots at -20<sup>o</sup>C
-|}