Yu:Dialysis: Difference between revisions
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|+'''SpectraPor Membrane Dialysis''' | |+'''SpectraPor Membrane Dialysis''' | ||
|valign="top"|1. Cut an appropriate length of dialysis tubing. You will need | |valign="top"|1. Cut an appropriate length of dialysis tubing. You will need 5cm for tying, plus 5cm tubing per 1mL. | ||
|- | |- | ||
|2. Soak in a large volume of distilled H<sub>2</sub>O for 30 minutes | |2. Soak in a large volume of distilled H<sub>2</sub>O for 30 minutes |
Revision as of 07:06, 18 May 2010
1. Cut an appropriate length of dialysis tubing. You will need 5cm for tying, plus 5cm tubing per 1mL. |
2. Soak in a large volume of distilled H2O for 30 minutes |
3. Soak in a large volume of 2% Sodium Bicarbonate (w/v), 1mM EDTA at 60°C for 30 minutes |
4. Rinse thoroughly in a large volume of distilled H2O. |
5. Carefully tie off one end of the tubing with a double-knot. |
6. Add solution to be dialyzed by pipetting. |
7. Clamp off the open end with a plastic floating clamp. |
8. Suspend in the dialysis solution in a large beaker or graduated cylinder, depending on the length of the dialysis tubing. |
9. Change dialysis solution as needed. |
10. When dialysis is complete, carefully pinch off the tubing under the clamp. Remove the clamp carefully - note that the tubing will break where it was clamped if you are not supporting the tubing. |
11. Carefully decant the dialyzed solution by pipetting or by pouring. |