http://openwetware.org/index.php?title=Yeast_Colony_PCR&feed=atom&action=history 2013-05-20T06:24:28Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=295397&oldid=prev 2009-03-20T22:59:03Z

<p><span class="autocomment">Lab Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:59, 20 March 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 4:</td> <td colspan="2" class="diff-lineno">Line 4:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Endy:Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Endy:Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR_v2.0|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR v2.0]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR_v2.0|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR v2.0]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Tetm2002 http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=295392&oldid=prev 2009-03-20T22:47:45Z

<p><span class="autocomment">Lab Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:47, 20 March 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Endy:Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Endy:Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Blackburn:<del class="diffchange diffchange-inline">Yeast_Colony_PCR</del>|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR v2.0]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[Blackburn:<ins class="diffchange diffchange-inline">Yeast_Colony_PCR_v2.0</ins>|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR v2.0]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]</div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Tetm2002 http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=295391&oldid=prev 2009-03-20T22:47:10Z

<p><span class="autocomment">Lab Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:47, 20 March 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Endy:Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Endy:Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR v2.0]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]</div></td></tr> <!-- diff generator: internal 2013-05-20 06:02:44 --> <!-- diff cache key owwdb:diff:version:1.11a:oldid:115693:newid:295391 --> </table>

Tetm2002 http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=115693&oldid=prev 2007-05-09T18:09:23Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:09, 9 May 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 7:</td> <td colspan="2" class="diff-lineno">Line 7:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Protocol]] </ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Yeast]] </ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:DNA]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Reshma P. Shetty http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=70100&oldid=prev 2006-09-13T00:03:55Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 00:03, 13 September 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[<del class="diffchange diffchange-inline">http://openwetware.org/wiki/</del>BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[<ins class="diffchange diffchange-inline">[</ins>BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4<ins class="diffchange diffchange-inline">]</ins>]</div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Josh K. Michener http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=70097&oldid=prev 2006-09-12T23:50:00Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:50, 12 September 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[http://openwetware.org/wiki/BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]</ins></div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Nkuldell http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=70060&oldid=prev 2006-09-12T22:04:59Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:04, 12 September 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 2:</td> <td colspan="2" class="diff-lineno">Line 2:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lab Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lab Protocols==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]<del class="diffchange diffchange-inline">&lt;br/&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*</ins>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Endy:Yeast Colony PCR]]<del class="diffchange diffchange-inline">&lt;br/&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*</ins>[[Endy:Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*</ins>[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy Yeast Colony PCR<ins class="diffchange diffchange-inline">]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)</ins>]]</div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Josh K. Michener http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=40963&oldid=prev 2006-06-12T04:33:13Z

<p><span class="autocomment">Lab Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 04:33, 12 June 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 2:</td> <td colspan="2" class="diff-lineno">Line 2:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lab Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lab Protocols==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]&lt;br&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]&lt;br<ins class="diffchange diffchange-inline">/</ins>&gt;</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Endy:Yeast Colony PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Endy:<ins class="diffchange diffchange-inline">Yeast Colony PCR]]&lt;br/&gt;</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Blackburn:Yeast_Colony_PCR|Blackburn Lab:&nbsp; Quick and Easy </ins>Yeast Colony PCR]]</div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Tetm2002 http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=17642&oldid=prev 2006-01-17T17:10:27Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:10, 17 January 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Back to [[Protocols]]''</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lab Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lab Protocols==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]&lt;br&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]&lt;br&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Endy:Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Endy:Yeast Colony PCR]]</div></td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Barry Canton http://openwetware.org/index.php?title=Yeast_Colony_PCR&diff=17639&oldid=prev 2006-01-17T17:08:43Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:08, 17 January 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 2:</td> <td colspan="2" class="diff-lineno">Line 2:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]&lt;br&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]&lt;br&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Endy:Yeast Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Endy:Yeast Colony PCR]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#Using sterile pipette tips, transfer a 1 mm colony into 50 uL of 60 U/ml Zymolase</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#*3 uL of 1 U/mL Zymolase stock solution</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#*47 uL of water</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#Incubate cell suspension at 37 C for 30 minutes.</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#Incubate cell suspension at 95 C for 10 minutes.</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#Use 5 uL of the cell suspension for a 50 uL PCR reaction</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Zymolase Stock Solution'''</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*10,000 U lyticase (Sigma)</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*1 mL KHPO4 buffer, pH 7.5 (adjusted with KOH)</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*0.2 mL 5M NaCl</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*5 mL 100% glycerol</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Add H2O to 10 mL</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Store at -20 C</del></div></td><td colspan="2">&nbsp;</td></tr> <!-- diff generator: internal 2013-05-20 06:24:29 --> </table>

Barry Canton