Yeast Colony PCR

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[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]<br>
[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]<br>
[[Endy:Yeast Colony PCR]]
[[Endy:Yeast Colony PCR]]
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#Using sterile pipette tips, transfer a 1 mm colony into 50 uL of 60 U/ml Zymolase
 
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#*3 uL of 1 U/mL Zymolase stock solution
 
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#*47 uL of water
 
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#Incubate cell suspension at 37 C for 30 minutes.
 
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#Incubate cell suspension at 95 C for 10 minutes.
 
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#Use 5 uL of the cell suspension for a 50 uL PCR reaction
 
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'''Zymolase Stock Solution'''
 
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*10,000 U lyticase (Sigma)
 
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*1 mL KHPO4 buffer, pH 7.5 (adjusted with KOH)
 
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*0.2 mL 5M NaCl
 
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*5 mL 100% glycerol
 
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*Add H2O to 10 mL
 
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*Store at -20 C
 

Revision as of 13:08, 17 January 2006

Lab Protocols

Silver Lab: Yeast Colony PCR
Endy:Yeast Colony PCR

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