http://openwetware.org/index.php?title=YT&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Sat, 25 May 2013 08:20:28 GMT http://openwetware.org/index.php?title=YT&diff=610824&oldid=prev <p>New page: == Summary == 2x YT Medium. == Ingredients == ===For 1000 ml=== *10g Bacto yeast extract *16g Bacto Tryptone *5g NaCl Adjust to pH 7.0 prior to use. ==Extended instructions== *combine:...</p> <p><b>New page</b></p><div>== Summary ==<br /> 2x YT Medium.<br /> <br /> == Ingredients ==<br /> ===For 1000 ml===<br /> *10g Bacto yeast extract<br /> *16g Bacto Tryptone<br /> *5g NaCl<br /> <br /> Adjust to pH 7.0 prior to use.<br /> <br /> ==Extended instructions==<br /> <br /> *combine:<br /> {| border=&quot;1&quot; cellpadding=&quot;5&quot; cellspacing=&quot;0&quot; <br /> |+ <br /> ! Reagent<br /> ! for 1 L<br /> ! 500 mL<br /> ! 100 mL<br /> |-=<br /> | tryptone <br /> | 16 g<br /> | 8 g<br /> | 1.6 g<br /> |-<br /> | yeast<br /> | 10 g<br /> | 5 g<br /> | 1 g<br /> |-<br /> | NaCl<br /> | 5 g<br /> | 2.5 g<br /> | 0.5 g<br /> |-<br /> | ddH2O <br /> | 980 ml<br /> | 490 ml<br /> | 98 ml<br /> |}<br /> *adjust pH to 7.0 w/ NaOH<br /> *bring to volume<br /> *Sterilize by autoclaving<br /> <br /> ==Source==<br /> Adapted From:<br /> <br /> F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley &amp; Sons, ed. 4, 1999) pg. A1-36<br /> <br /> ==Trivia==<br /> <br /> [[Category:Material]] [[Category:Standard Media]]</div> Thu, 28 Jun 2012 08:53:23 GMT Christopher C Vanlang http://openwetware.org/wiki/Talk:YT