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'''X-Laboratory'''  </font> <br>


(跨膜与感觉信号转导研究组) <br>
'''X-Laboratory for Ion-Channel Engineering'''  </font> <br>
<br>
 
<font face="Helvetica" size=5> (离子-通道工程研究组) </font> <br>
 
[http://ygy.buaa.edu.cn Beijing Advanced Innovation Center for Biomedical Engineering]
 
[http://bme.buaa.edu.cn School of Biological Science and Medical Engineering, Beihang University]
 
<!--
[http://www.buaa.edu.cn School of Engineering Medicine, Beihang University]
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Principle Investigator: [http://openwetware.org/wiki/User:Xiaodong_Liu Xiaodong LIU, Ph.D. (刘晓冬)]
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From <font face="Helvetica" size=5>'''Scientific Discovery''' </font> of Ion Channels to <font face="Helvetica" size=5>'''Engineering Design''' </font> of Bioactuators and Biosensors
 
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[http://bme.med.tsinghua.edu.cn Department of Biomedical Engineering], [http://www.tsinghua.edu.cn/docsn/yxy/English/index_eng.html School of Medicine]
Principle Investigator: [http://openwetware.org/wiki/User:Xiaodong_Liu Xiaodong LIU, Ph.D.] [https://bme.buaa.edu.cn/teacherInfo.aspx?catID=7&subcatID=35&curID=13901 (刘晓冬)]
{{-}}
[http://life.tsinghua.edu.cn/english School of Life Sciences]  
{{-}}
at [http://www.tsinghua.edu.cn/eng/index.jsp Tsinghua University]


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[[X-Lab|Home]]|[[X-Lab|主页]]
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实验室简介 （LAB DESCRIPTION）
 
 
 
离子通道作为“生命系统中的晶体管”，通过调控胞内外离子动态交换等方式密切参与生命活动。本实验室主要关注离子通道及离子信号，尤其是钙离子通道（电压门控型CaV和瞬时受体电位型TRP）以及钙离子信号，深入理解 CaV1及TRPP激励-构象-门控-信号（Stimulus-Structure-Gating-Signaling）间的机制关联。
 
践行“分子生物医学工程”这一重要学科发展方向和趋势，本实验室秉承(生物医学)工程的理念和方法，致力于通过生物原理以及工程原理将生物元件、外源因子与兴奋性细胞膜相结合以重建/合成离子通道复合体的动态互作/组成及病/生理功能。上述的生物原件包括通道亚基、调制因子、信号蛋白和电解质等；常用的外源因子为小分子化合物、材料/力场、光电器件和计算机/单元等。
 
离子通道能够感知跨膜电压、离子信号、机械力及电磁辐射等多种模态外源性激励，这些非凡能力令人称奇，然而离子通道又是怎样在亚分子、分子及细胞水平上传感、转换及编码这些激励的能量和信息呢？综合运用电生理、生化、分子生物学及光学成像，以及生物物理建模、工程设计及分析等多种方法，我们以发现离子通道与离子信号的新机制、新现象和重要病生理为己任，同时基于离子通道开拓生物医学工程创新和物质世界新认知。
 
 
当前的具体研究方向包括:
 
1) 钙通道/信号蛋白复合体结构-功能研究（CaV1及TRPP）；
 
2) 钙振荡及特殊钙响应的功能及机制（CaV1及TRPP）；
 
3）兴奋性细胞的可塑性：钙通道/钙信号活动依赖性的分子及细胞机制（CaV1)；
 
4）利用这些基础研究的新进展，开发离子-通道相关的新工具、新方法和新仪器，催生新的发现，推动新的应用 (CaV1及TRPP)。
 
 
 
Our research has been in the field of ion channels and ionic signaling, mainly focusing on calcium channels (voltage-gated calcium channels CaV and transient receptor potential channels TRP) and calcium signaling.  Major interests are on in-depth gating mechanisms in relation with cell signaling (the couplings of Stimulus-Structure-Gating-Signaling) for CaV1 and TRPP channels.  We are particularly attracted by the amazing capabilities of the ion-channel system, curious about how exactly the stimuli (i.e., energy/information) are transduced or encoded at atomic (e.g., ions), molecular (e.g., proteins) and cellular (e.g., neurons) levels?  The methodologies utilized in the lab include electrophysiology, optical imaging, molecular & structural biology, biophysical modeling, and engineering design & analysis. Our mission is to discover new phenotypes & mechanisms and key pathological relevance, meanwhile to develop de novo tools/methods based on our scientific discoveries. 
 
Exemplary projects currently being pursued by students/postdocs include:
 
 
 
 
1) structure-function analysis of voltage- and ligand-gated calcium channels;
 
 
2) molecular/cellular information processing by ion-channel system such as electrical/calcium oscillations in neurons;
 
 
3）plasticity of excitable cells, e.g., channel activity-gene transcription coupling mediated by calcium channels and calcium signals
 
 
4）de novo tool, methods and instruments based on scientific discoveries on ion-channels, expected to foster new discoveries and applications.
 
 
</font><br>
 
 
 
<!--
Our research has been in the field of '''ion channels and signaling''', focusing on '''subthreshold or non-spiking behaviors''' and favoring concepts and methods from '''synthetic electrophysiology'''.  Major interests are on in-depth gating mechanisms in relation with cell signaling (gating-signaling), pursued by applying both biological and engineering principles onto biological entities (channel subunits, physiological ions, modulators and signaling proteins) and physical components (electrical & optical materials, devices and instruments) coupled with excitable membranes for the reconstitution of ion channels’ functionality & pathophysiology.  We are particularly attracted by the amazing capabilities of ion channels in sensing exogenous stimuli of diverse modality, such as transmembrane potentials, ionic signals, mechanical forces and electromagnetic radiations.  How exactly would ion channels sense, transduce and encode the stimuli (i.e., energy and information) at submolecular, molecular and cellular levels?  Based on these core biophysics, we pursue mechanistic understanding, pathophysiological ramifications and bioengineering innovations, of '''ionic signaling''' via ion channels.  Particularly, our research direction at the cellular/neuronal level is '''information processing''': sensory signaling and neural oscillations, and the other major direction is about '''plasticity''': excitation-transcription coupling and calcium channels/signals.
The methodologies span from electrophysiology, biochemistry, molecular biology and optical imaging to biophysical modeling and engineering design & analysis.  We are also interested in developing ''de novo'' tools/methods when necessary and possible for targeted sciences.
 
离子通道作为“生命系统中的晶体管”，通过调控胞内外离子动态交换等方式密切参与生命活动。离子通道能够响应跨膜电压、化学物质、机械力及电磁辐射等多种模态外源性激励，这些非凡能力令人称奇，然而离子及通道又是怎样在细胞、分子甚至原子水平上感知、转换及编码这些激励的能量和信息呢？实验室谋求离子通道与离子信号的新机制、重要病生理关联及生物工程创新。实验室常用的方法学涉及从电生理、生化、分子生物学及光学成像到生物物理建模、工程设计及分析等多个方面。当科学问题涉及到并且必要时，我们也有兴趣发展创立新的工具和方法。
 
本实验室的研究聚焦于钙离子通道（电压门控型CaV和瞬时受体电位型TRP）以及钙离子信号，深入理解 CaV1及TRPP激励-构象-门控-信号（Stimulus-Structure-Gating-Signaling）间的机制关联。基于(生物医学)工程的理念和方法，致力于通过生物原理以及工程原理将生物元件、外源因子与兴奋性细胞膜相结合以重建（合成）离子通道的功能及病生理。上述的生物原件包括通道亚基、调制因子及信号蛋白；常用的外源因子为化合物、材料、光电器件等。具体研究方向：1）在分子和细胞水平上的信息处理：感觉信号及神经振荡；2）兴奋性细胞的可塑性：“兴奋-转录-突起发生”耦联及钙通道/钙信号；3）基于科学新发现，开发离子-通道相关的新工具和新方法，催生新的科学发现，以及新的仪器和应用。
 
 
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[[Image:reprework_X2021_Chronic.jpg|480px]]
 
 
<!--[https://www.biorxiv.org/content/10.1101/2022.01.09.475579v1 Chronic Ca2+ imaging of cortical neurons with long-term expression of GCaMP-X (bioRxiv preprint 2021)] -->
 
[https://elifesciences.org/articles/76691 Chronic Ca2+ imaging of cortical neurons with long-term expression of GCaMP-X (2022)]
 
'''GCaMP7-X''' plasmids are now available through [https://www.addgene.org addgene]!  </font>
 
pEGFP-N1-GCaMP7b-XC , ID: 178361 [https://www.addgene.org/178361].
 
pEGFP-N1-GCaMP7b-XN , ID: 178362 [https://www.addgene.org/178362].
 
 
 
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<div style="clear: left; text-align: left; float: none">
 
 
[[Image:reprework_X2022_CaV1-neurito1.png|480px]]
 
 
[https://www.nature.com/articles/s42003-022-03438-1 Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling (2022) ]
 
'''DCT peptides''' plasmids are now available through [https://www.addgene.org addgene]!  </font>
 
NLS-CCTD , ID: 184325.
 
NES-CCTD , ID: 184326.
 
NLS-CCTC , ID: 184327.
 
NES-CCTC , ID: 184328.
 
 
 
 
</div>
 
 
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[[Image:reprework_X2018_GCaMP-X.JPG|600px]]
 
[https://www.nature.com/articles/s41467-018-03719-6 Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP (2018)]
 
 
<font face="Helvetica" size=6 color=#339900>
 
 
[https://f1000.com/prime/733057566?bd=1 F1000 Recommended!]
 
 
 
NOW GCaMP6-X plasmid is available through [https://www.addgene.org/browse/article/28193453/ addgene]!  </font>
 
pEGFP-N1-GCaMP6m-XC , ID: 111543.
 
pEGFP-N1-GCaMP6m-XN , ID: 118976.
 
AAV-syn-GCaMP6m-XC , ID: 118975.
 
 
<font face="Helvetica" size=5>
 
 
现在GCaMP6-X质粒可通过[http://www.sinozhongyuan.com  北京中原公司（addgene代理）]获取！
 
 
相关病毒rAAV-hSyn-GCaMP6m-XC-WPRE-hGH polyA （产品编号 PT-1464）！
 
 
 
[[Image:GCaMP-X_2020_Sum.JPG|400px]]
 
GCaMP-X相关质粒全球索取情况 （不完全统计，2020.2更新）
 
 
Global requests of GCaMP-X plasmids （incomplete summary，updated 2020.2）
 
 
 
</font>
 


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LAB DESCRIPTION


Our lab is broadly interested in transmembrane and sensory signaling based on channels, pursuing both biophysical mechanisms and bioengineering innovations.
---------------------------------


One of the most important signaling pathways to convey information from external world into biological systems is by way of channels sitting across the membrane. Such channels: could be cued either by physical (e.g. voltage or photons) or by chemical signals (e.g. toxins or ions); could be either natural channels (e.g. ion channels conductive to Ca<sup>2+</sup> or K<sup>+</sup>) or engineered channels (e.g., nanopores by ultrasound). We mainly focus on fundamental mechanisms critical to channel complexes involved in transmembrane signaling, especially those related to sensory functions, such as vision, taste, hearing and other less-studied modalities. Representative work toward this direction refers to [http://www.nature.com/nature/journal/v463/n7283/full/nature08766.html '''Liu X.''' ''et. al.'' '''Nature''']. Meanwhile, we actively explore the potentials of novel methodologies developed or derived from our basic research, such as sonoporation, channel modulators, biomolecular sensors etc. [http://openwetware.org/wiki/User:Xiaodong_Liu#Publications relevant publications]
---------------------------------




</font><br><br>


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[[Image:Reprework_X2018_PKD2L1_Structure_NC.png|360px]]


== Lab News ==


'"X-Lab Students attended Tsinghua BoShiShen Symposium of Medical School, Nov 19, 2011, Beijing'''
[https://www.nature.com/articles/s41467-018-03606-0 Cryo-EM structure of the polycystic kidneydisease-like channel PKD2L1 2018]


JIANG Peng (Prize for Excellent Oral Talks)


LIU Nan, et al. (Poster)
[https://www.rcsb.org/structure/5Z1W PDB accession of PKD2L1 structure: 5Z1W]


Ji Tang, et al. (Poster)


</div>




'''JIANG Peng from X-Lab received "excellent poster award" at 17th “International Biophysics Congress” (IAPUB)  Oct 30-Nov 3 Beijing, China
--------------------------
'''
Congratulations, Peng!


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'''LIU Xiaodong presented X-Lab research as an invited talk at Asia Communications and Photonics Conference (ACP2011), Nov. 13-16, 2011, Shanghai
'''


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'''JIANG Peng from X-Lab presented research at Tsinghua BoShiShen Symposium of Life and Biomedical Sciences, Oct 15-16, 2011, Beijing
[[Image:reprework_X2017_CMI_eLife.png|350px]]
'''




[http://elifesciences.org/content/6/e21989 Cooperative and acute inhibition by multiple C-terminal motifs of L-type Ca2+ channels (2017)]


[[Image:JIANGPeng2011.jpg|thumb|left|100x100px]]


{{-}}
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CMI-related plasmids are available through [https://www.addgene.org/Xiaodong_Liu/ addgene]!  </font>


'''X-Lab presented research at CaBP17, July 17-20, 2011, Beijing
<font face="Helvetica" size=4>
'''


LIU Nan ''et al.''(poster)


XU Yanyan ''et al.'' (poster)
CMI相关质粒可通过[http://www.sinozhongyuan.com  北京中原公司（addgene代理）]获取！ </font>


LIU Xiaodong （invited talk)




</div>


'''The X-lab is starting to accept PTN students for research rotations!'''


http://life.tsinghua.edu.cn/home/announce/1613.html
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http://www.nibs.ac.cn/?act=view&id=2741
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'''Postdoctoral Position Openings'''
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http://www.nature.com/naturejobs/science/jobs/191089-Postdoctoral-Fellow-Tsinghua-University




[[Image:reprework_X2016_1.png|250px]]


'''X-Lab (LIU Qing, XIE Xin and LIU Xiaodong) presented the poster:


CHARACTERIZATION OF QUANTITATIVE FRET SENSORS FOR FLUORESCENCE MOLECULAR TOMOGRAPHY'''
[http://www.ncbi.nlm.nih.gov/pubmed/27362349 IVS3-S4 modulating CaV1 voltage-gated activation by linker flexibility (2016)]


Author Block: Qing Liu, Xin Xie, Yi Zhang, Yanyan Xu, Yue Zhang, Xin Liu, Jing Bai, Xiaodong Liu.
</div>


Tsinghua University, Beijing, China.


during Biophysics Society Annual Meeting, March 2011 at Baltimore, MD, USA
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== Updates ==
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Individual Meetings (Starting from 2011/10)
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<!--
[[Image:reprework_X2015_2.png|600px]]
[[Image:MeetingsXLab201108.jpg|thumb|left|800x300px|X-LAB Schedule]]  
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[http://www.cell.com/cell-reports/abstract/S2211-1247(15)01066-9 TRPP transient sensing and mechanisms through dual Ca2+ feedbacks (2015)]


17th “International Biophysics Congress” (IAPUB)  Oct 30-Nov 3 Beijing, China
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http://www.17ibc.org/


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55th Annual Meeting of Biophysical Society March 5 - 9, 2011


http://www.biophysics.org/2012meeting


<font face="Helvetica" size=4>
<font face="Helvetica" size=4>
[[X:News|张雯同学参加2024年中国生物学学会女性科学家论坛并获得优秀墙报奖 2024.3]]
[[X:News|X-Lab PhD student GENG Jinli has been selected as a Student Travel Award recipient to present the work at 2024 Biophysical Society Annual Meeting (Philadelphia USA), congratulations!]]  [https://www.biophysics.org/news-room/biophysical-society-names-2024-graduate-travel-award-recipients 2024.2]
[[X:News|宾州州立大学（PSU）Donald Gill教授来访并作学术报告]] [https://openwetware.org/wiki/File:2023Gill_Beihang.JPG 2023.10]
[[X:News|刘晓冬教授近日受聘担任：中国生物物理学会 钙信号分会 委员、中国生物医学工程学会 生物医学测量分会 副主任委员 2023.8]]
[[X:News|刘晓冬教授在2023中国生物物理年会钙信号分会(长沙)做口头学术报告 2023.7]]
[[X:News|X-Lab于振同学在2023全国离子通道与受体青年会议(成都)做口头学术报告]]  [https://openwetware.org/wiki/File:2023YU_Chengdu.JPG 2023.6]
[[X:News|X-Lab耿金丽同学在2023IFMBE(苏州)会议上荣获青年优秀论文一等奖]]  [https://openwetware.org/wiki/File:2023GENG_Jinli.JPG 2023.5]
[[X:News|X-Lab在《eLife》发表钙探针及成像研究新进展，祝贺共同第一作者耿金丽和于振同学！ 2022.10]]  [https://elifesciences.org/articles/76691 eLife]  [https://news.buaa.edu.cn/info/1005/57054.htm  北航新闻]  [https://mp.weixin.qq.com/s/lGaYqWrZAFVGcxsC80pbpg 科学媒体报道] [https://mp.weixin.qq.com/s/5mVPdjAQAgq6G_-moGHGqw 清华麦戈文新闻]
[[X:News|X-Lab耿金丽同学在全国青年离子通道（武汉）会议上做口头报告 2022.7]]
[[X:News|X-Lab在《通讯-生物学》发表神经元钙通道-钙信号研究新进展，祝贺共同第一作者杨亚雄博士及于振、耿金丽同学！ 2022.5]]  [https://www.nature.com/articles/s42003-022-03438-1 Communications Biology]  [http://news.buaa.edu.cn/info/1005/56246.htm  北航新闻] [https://www.tsinghua.edu.cn/info/1181/95238.htm 清华新闻] [https://mp.weixin.qq.com/s/0-VuqvF394RPbS8G63ReLA 科学媒体报道]
[[X:News|X-Lab耿金丽同学在中国生物医学工程大学生医测量分会场做口头报告 2022.4]]
[[X:News|X-Lab高蕴溟、黎文祥同学顺利通过硕士论文答辩，祝贺！2022.1]]
[[X:News|高蕴溟、黎文祥同学合作展示的工作获得第五届全国青年离子通道会议优秀墙报奖 2021.4]]
[[X:News|X-Lab杨亚雄博士(直博PhD 12-18, 博后Postdoc 18-20)获聘北航生物与医学工程学院准聘副教授，祝贺！ 2020.11]]
[[X:News|左佳琪同学在X-Lab完成的本科毕设工作评为北航生医学院2020优秀毕业论文 2020.6]]
[[X:News|X-LAB获得北京市自然科学基金资助: 重点（医学 刘晓冬）及 青年（生物科学 杨亚雄）各一项 2020.1]]
[[X:News|X-LAB获得国家自然科学基金资助：面上（医学部，刘晓冬）及 青年（数理学部，杨亚雄）各一项 2019.8]]
[[X:News|X-LAB博士生获得国际离子通道大会(7th IICC, Hangzhou China) excellent poster award 2019.6]]
[[X:News|黎文祥同学在X-Lab完成的本科毕设工作评为北航生医学院2019优秀毕业论文 2019.6]]
[[X:News|X-LAB博士后杨亚雄获得中国博士后基金第64批面上资助 2018.11]] [http://news.sciencenet.cn/htmlnews/2018/11/419892.shtm 公示名单] 
[[X:News|X-LAB博士生获国际钙信号与细胞功能（12th SCSC）会议 Best poster award 2018.7]]
[[X:News|X-LAB获清华大学优秀博士学位论文奖 2018.7]]
[[X:News|X-LAB博士生杨亚雄顺利通过博士论文答辩，并入选“中国博士后创新人才支持计划”，祝贺！ 2018.5 ]] [http://www.edu.cn/rd/gai_kuang/xin_wen_gong_gao/201805/t20180510_1599429.shtml 2018博新计划公示名单]
[[X:News|X-LAB杨亚雄、刘楠、何元源合作完成的工作在线发表于《Nature Communications》2018.4]] [https://www.nature.com/articles/s41467-018-03719-6 Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP] [http://www.med.tsinghua.edu.cn/SingleServlet?newsId=985 Tsinghua Med News]
[[X:News|X-LAB与清华大学结构生物学中心研究人员合作完成的工作在线发表于《Nature Communications》2018.3]] [https://www.nature.com/articles/s41467-018-03606-0 Cryo-EM structure of the polycystic kidneydisease-like channel PKD2L1]
[http://www.med.tsinghua.edu.cn/SingleServlet?newsId=945 Tsinghua Med News]


<!--
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Journal Club (Jointed with DU Lab and SONG Lab)
[[X:News|X-LAB博士生在2017年10月举行的中国生理学会青年会议上获得优秀口头报告奖（杨亚雄）及优秀墙报奖（刘玉霞），祝贺！]]
 
-->
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[[X:News|X-LAB博士生刘楠（直博PhD 11-17）顺利通过博士论文答辩，并即将赴云南大学生命学院任教，祝贺！ 2017.6]]
[[X:News|X-LAB博士生刘楠、杨亚雄、葛琳合作完成的工作在线发表于《eLife》2017.1]] [http://elifesciences.org/content/6/e21989 Cooperative and acute inhibition by multiple C-terminal motifs of L-type Ca2+ channels] [http://news.tsinghua.edu.cn/publish/thunews/10303/2017/20170109130039430720206/20170109130039430720206_.html Tsinghua NEWS]
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[[X:News|X-LAB博士生刘楠关于L型电压门控钙通道的工作在线发表于《Channels》2016.6]] [http://www.ncbi.nlm.nih.gov/pubmed/27362349 Linker flexibility of IVS3-S4 loops modulates voltage-dependent activation of L-type Ca2+ channels]




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[[X:News|X-LAB学生杨亚雄、葛琳同学在清华大学麦戈文研究所retreat中分获最佳提问、优秀墙报等奖项 2016.6]] 


<!--== 2010 ==


[[X:News|X-LAB博士生在2015年中国生理学会青年会议上获得优秀论文奖（1st Place）2015.10]]  [http://bme.med.tsinghua.edu.cn/index.php?option=com_content&task=view&id=661&Itemid=90 BME Departmental NEWS]


ZHANG Yue 8/12 FRET-based FMT


XU Yanyan 9/10 GPCR/Sensory Neuron
[http://www.cell.com/cell-reports/abstract/S2211-1247(15)01066-9 X-LAB关于TRPP通道传感放大机制在线发表于Cell Reports 2015.10] [http://news.tsinghua.edu.cn/publish/thunews/9659/2015/20151016144339053389179/20151016144339053389179_.html Tsinghua NEWS]


ZHAO Shan (from Du Lab) 9/25 Remotely Triggerable Drug Delivery Systems


LIU Qing  10/15 reversible photoswitchable rsTagRFP for pcFRET
[[X:News|X-LAB博士生在2015年中以双边离子通道学术会议上获得Excellent Poster Award]]


YU Tao (from Song Lab) 10/22 Tetracycline-regulatable factors with distinct dimerization
domains allow reversible growth inhibition by p16


TSE Yan Franco (XIE Xin)  10/29 Calcium Channels
[[X:News|X-LAB本科毕业设计获评2014年清华大学优秀毕业论文]]


NIU Pengxia (from DU Lab) 11/5 fuorescent structural DNA nanoballs functionalized with phsphate-linked nuceotide triphosphates


LI Xuesong (from SONG Lab) 11/19 global and local fmri signals driven by neurons defined optogenetically by type and writing
[[X:News|X-LAB博士生在2014第十届全国钙信号会议(CSCS)上获评最佳墙报奖]]
Claudia Wittkowske (from DU Lab) 11/26 "Glioblastoma stem-like cells give rise to tumour endothelium"


XUE Wenwen 12/10 bacteria magnetosome


YU Tao (from SONG Lab) 12/17 optical dimerization
[[X:News|X-LAB本科毕业设计获得清华学堂班2014最佳科研成果奖]]


XU Yanyan 12/24 Functional connectivity in the retina at the resolution of photoreceptors
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<!--== 2011 ==
[[X:News|X-LAB博士生获得清华大学短期出国访学基金]]




ZHAO Shan (from Du Lab) 1/7 Mimicking nature by codelivery of stimulant and inhibitor to create temporally stable and spatially restricted angiogenic zones
[[X:News|X-LAB博士后获评CLS优秀博士后基金]]


LI Xuesong (from SONG Lab) 2/25 "Genetic dissection of an amygdala microcircuit that gates conditioned fear" and  "Encoding of conditioned fear in central amygdala inhibitory circuits"


LIU Nan 3/28 Salty Taste
[[X:News|X-LAB博士后获评54期中国博士后基金]]




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[[X:News|X-LAB本科生获评2013年优秀毕业论文]]


[[Image:X.png|thumb|left|400x400px|X-LAB. ]]


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[[X:News|X-LAB硕士生获得2011年国际生物物理年会Best Poster Award]]
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[[X:BS20013|Attention: 刘晓冬研究组（X-LAB）本科生研究课题]]
<br>The following positions are currently available:


'''Postdoctoral Fellows'''
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'''Ph.D. Students'''
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'''Masters' Students'''
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Please send email to [mailto:liuX.tsinghua@gmail.com Dr. Liu] if you are interested. Include your CV and a brief description of your career goals.
[http://www.ncbi.nlm.nih.gov/pubmed/20139964 Competitive tuning and mechanisms of CaV1 channels mediated by calmodulin and distal carboxyl tail]
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We also welcome '''undergraduate students''' (especially from major research universities in Beijing) to conduct research in our lab on thesis projects.
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All above positions can start immediately.
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【2018.08】本实验室正在招募博士后，欢迎对本实验室研究感兴趣的候选人同我们联系。详情请参见[http://ygy.buaa.edu.cn/info/1003/1241.htm 北航医工院招聘启事]。




【2013.07.09】本实验室正在招募博士生、博士后（及技术员），欢迎对本实验室研究感兴趣、并希望在生物医学工程领域的科学研究上有所作为的候选人同本实验室PI [http://openwetware.org/wiki/User:Xiaodong_Liu Xiaodong LIU, Ph.D. (刘晓冬)]联系。另请参见[http://talent.sciencenet.cn/info.aspx?id=12706 科学网的招聘广告]。
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Latest revision as of 01:01, 13 March 2024




X-Laboratory for Ion-Channel Engineering

(离子-通道工程研究组)

Beijing Advanced Innovation Center for Biomedical Engineering

School of Biological Science and Medical Engineering, Beihang University


From Scientific Discovery of Ion Channels to Engineering Design of Bioactuators and Biosensors

Principle Investigator: Xiaodong LIU, Ph.D. (刘晓冬)



Welcome to the X-Lab!


实验室简介 （LAB DESCRIPTION）


离子通道作为“生命系统中的晶体管”，通过调控胞内外离子动态交换等方式密切参与生命活动。本实验室主要关注离子通道及离子信号，尤其是钙离子通道（电压门控型CaV和瞬时受体电位型TRP）以及钙离子信号，深入理解 CaV1及TRPP激励-构象-门控-信号（Stimulus-Structure-Gating-Signaling）间的机制关联。

践行“分子生物医学工程”这一重要学科发展方向和趋势，本实验室秉承(生物医学)工程的理念和方法，致力于通过生物原理以及工程原理将生物元件、外源因子与兴奋性细胞膜相结合以重建/合成离子通道复合体的动态互作/组成及病/生理功能。上述的生物原件包括通道亚基、调制因子、信号蛋白和电解质等；常用的外源因子为小分子化合物、材料/力场、光电器件和计算机/单元等。

离子通道能够感知跨膜电压、离子信号、机械力及电磁辐射等多种模态外源性激励，这些非凡能力令人称奇，然而离子通道又是怎样在亚分子、分子及细胞水平上传感、转换及编码这些激励的能量和信息呢？综合运用电生理、生化、分子生物学及光学成像，以及生物物理建模、工程设计及分析等多种方法，我们以发现离子通道与离子信号的新机制、新现象和重要病生理为己任，同时基于离子通道开拓生物医学工程创新和物质世界新认知。


当前的具体研究方向包括:

1) 钙通道/信号蛋白复合体结构-功能研究（CaV1及TRPP）；

2) 钙振荡及特殊钙响应的功能及机制（CaV1及TRPP）；

3）兴奋性细胞的可塑性：钙通道/钙信号活动依赖性的分子及细胞机制（CaV1)；

4）利用这些基础研究的新进展，开发离子-通道相关的新工具、新方法和新仪器，催生新的发现，推动新的应用 (CaV1及TRPP)。


Our research has been in the field of ion channels and ionic signaling, mainly focusing on calcium channels (voltage-gated calcium channels CaV and transient receptor potential channels TRP) and calcium signaling. Major interests are on in-depth gating mechanisms in relation with cell signaling (the couplings of Stimulus-Structure-Gating-Signaling) for CaV1 and TRPP channels. We are particularly attracted by the amazing capabilities of the ion-channel system, curious about how exactly the stimuli (i.e., energy/information) are transduced or encoded at atomic (e.g., ions), molecular (e.g., proteins) and cellular (e.g., neurons) levels? The methodologies utilized in the lab include electrophysiology, optical imaging, molecular & structural biology, biophysical modeling, and engineering design & analysis. Our mission is to discover new phenotypes & mechanisms and key pathological relevance, meanwhile to develop de novo tools/methods based on our scientific discoveries.

Exemplary projects currently being pursued by students/postdocs include:



1) structure-function analysis of voltage- and ligand-gated calcium channels;


2) molecular/cellular information processing by ion-channel system such as electrical/calcium oscillations in neurons;


3）plasticity of excitable cells, e.g., channel activity-gene transcription coupling mediated by calcium channels and calcium signals


4）de novo tool, methods and instruments based on scientific discoveries on ion-channels, expected to foster new discoveries and applications.








Chronic Ca2+ imaging of cortical neurons with long-term expression of GCaMP-X (2022)

GCaMP7-X plasmids are now available through addgene!

pEGFP-N1-GCaMP7b-XC , ID: 178361 [1].

pEGFP-N1-GCaMP7b-XN , ID: 178362 [2].






Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling (2022)

DCT peptides plasmids are now available through addgene!

NLS-CCTD , ID: 184325.

NES-CCTD , ID: 184326.

NLS-CCTC , ID: 184327.

NES-CCTC , ID: 184328.






Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP (2018)



F1000 Recommended!


NOW GCaMP6-X plasmid is available through addgene!

pEGFP-N1-GCaMP6m-XC , ID: 111543.

pEGFP-N1-GCaMP6m-XN , ID: 118976.

AAV-syn-GCaMP6m-XC , ID: 118975.



现在GCaMP6-X质粒可通过北京中原公司（addgene代理）获取！


相关病毒rAAV-hSyn-GCaMP6m-XC-WPRE-hGH polyA （产品编号 PT-1464）！


GCaMP-X相关质粒全球索取情况 （不完全统计，2020.2更新）


Global requests of GCaMP-X plasmids （incomplete summary，updated 2020.2）













张雯同学参加2024年中国生物学学会女性科学家论坛并获得优秀墙报奖 2024.3


X-Lab PhD student GENG Jinli has been selected as a Student Travel Award recipient to present the work at 2024 Biophysical Society Annual Meeting (Philadelphia USA), congratulations! 2024.2


宾州州立大学（PSU）Donald Gill教授来访并作学术报告 2023.10


刘晓冬教授近日受聘担任：中国生物物理学会 钙信号分会 委员、中国生物医学工程学会 生物医学测量分会 副主任委员 2023.8


刘晓冬教授在2023中国生物物理年会钙信号分会(长沙)做口头学术报告 2023.7


X-Lab于振同学在2023全国离子通道与受体青年会议(成都)做口头学术报告 2023.6


X-Lab耿金丽同学在2023IFMBE(苏州)会议上荣获青年优秀论文一等奖 2023.5


X-Lab在《eLife》发表钙探针及成像研究新进展，祝贺共同第一作者耿金丽和于振同学！ 2022.10 eLife 北航新闻 科学媒体报道 清华麦戈文新闻


X-Lab耿金丽同学在全国青年离子通道（武汉）会议上做口头报告 2022.7


X-Lab在《通讯-生物学》发表神经元钙通道-钙信号研究新进展，祝贺共同第一作者杨亚雄博士及于振、耿金丽同学！ 2022.5 Communications Biology 北航新闻 清华新闻 科学媒体报道


X-Lab耿金丽同学在中国生物医学工程大学生医测量分会场做口头报告 2022.4


X-Lab高蕴溟、黎文祥同学顺利通过硕士论文答辩，祝贺！2022.1


高蕴溟、黎文祥同学合作展示的工作获得第五届全国青年离子通道会议优秀墙报奖 2021.4


X-Lab杨亚雄博士(直博PhD 12-18, 博后Postdoc 18-20)获聘北航生物与医学工程学院准聘副教授，祝贺！ 2020.11


左佳琪同学在X-Lab完成的本科毕设工作评为北航生医学院2020优秀毕业论文 2020.6


X-LAB获得北京市自然科学基金资助: 重点（医学 刘晓冬）及 青年（生物科学 杨亚雄）各一项 2020.1


X-LAB获得国家自然科学基金资助：面上（医学部，刘晓冬）及 青年（数理学部，杨亚雄）各一项 2019.8


X-LAB博士生获得国际离子通道大会(7th IICC, Hangzhou China) excellent poster award 2019.6


黎文祥同学在X-Lab完成的本科毕设工作评为北航生医学院2019优秀毕业论文 2019.6


X-LAB博士后杨亚雄获得中国博士后基金第64批面上资助 2018.11 公示名单


X-LAB博士生获国际钙信号与细胞功能（12th SCSC）会议 Best poster award 2018.7


X-LAB获清华大学优秀博士学位论文奖 2018.7


X-LAB博士生杨亚雄顺利通过博士论文答辩，并入选“中国博士后创新人才支持计划”，祝贺！ 2018.5 2018博新计划公示名单


X-LAB杨亚雄、刘楠、何元源合作完成的工作在线发表于《Nature Communications》2018.4 Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP Tsinghua Med News


X-LAB与清华大学结构生物学中心研究人员合作完成的工作在线发表于《Nature Communications》2018.3 Cryo-EM structure of the polycystic kidneydisease-like channel PKD2L1 Tsinghua Med News


X-LAB博士生刘楠（直博PhD 11-17）顺利通过博士论文答辩，并即将赴云南大学生命学院任教，祝贺！ 2017.6


X-LAB博士生刘楠、杨亚雄、葛琳合作完成的工作在线发表于《eLife》2017.1 Cooperative and acute inhibition by multiple C-terminal motifs of L-type Ca2+ channels Tsinghua NEWS





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