X-Gal - Revision history

Christopher C Vanlang at 21:22, 23 December 2011

Christopher C Vanlang — Fri, 23 Dec 2011 21:22:30 GMT

←Older revision		Revision as of 21:22, 23 December 2011
Line 5:		Line 5:
	==Protocols using X-Gal==		==Protocols using X-Gal==
	*[[LacZ staining of cells]]		*[[LacZ staining of cells]]
		+
		+	==Notes==
		+	*Also may make stock solution with 50 mg in about 250 µl DMSO

	==See also==		==See also==

Jakob Suckale: external links

Jakob Suckale — Tue, 10 Jun 2008 15:38:26 GMT

external links

←Older revision		Revision as of 15:38, 10 June 2008
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	*[[Beta-galactosidase assay]]		*[[Beta-galactosidase assay]]

-		+	==External links==
		+	* S-Gal - a variation of X-Gal [http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Life_Science_Innovations/April_2001/April_2001_Feature_Article.html]

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Jakob Suckale: warnings

Jakob Suckale — Tue, 10 Jun 2008 15:38:15 GMT

warnings

←Older revision		Revision as of 15:38, 10 June 2008
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-	X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening.	+	X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues.

-	A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml.	+	A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation.

	==Protocols using X-Gal==		==Protocols using X-Gal==
Line 8:		Line 8:
	==See also==		==See also==
	*[[Beta-galactosidase assay]]		*[[Beta-galactosidase assay]]
		+
		+

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Jakob Suckale: links

Jakob Suckale — Tue, 10 Jun 2008 15:34:46 GMT

links

←Older revision		Revision as of 15:34, 10 June 2008
Line 2:		Line 2:

	A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml.		A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml.
		+
		+	==Protocols using X-Gal==
		+	*[[LacZ staining of cells]]
		+
		+	==See also==
		+	*[[Beta-galactosidase assay]]

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Austin J. Che: New page: X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for beta-galactosidase (lacZ) and turns blue upon being cleaved. It is commo...

Austin J. Che — Thu, 03 Apr 2008 22:35:37 GMT

New page: X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for beta-galactosidase (lacZ) and turns blue upon being cleaved. It is commo...

New page

X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening.

A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml.

[[Category:Material]] [[Category:Chemical]]