X-Gal
From OpenWetWare
(Difference between revisions)
(warnings) |
Current revision (17:22, 23 December 2011) (view source) m |
||
| (One intermediate revision not shown.) | |||
| Line 5: | Line 5: | ||
==Protocols using X-Gal== | ==Protocols using X-Gal== | ||
*[[LacZ staining of cells]] | *[[LacZ staining of cells]] | ||
| + | |||
| + | ==Notes== | ||
| + | *Also may make stock solution with 50 mg in about 250 µl DMSO | ||
==See also== | ==See also== | ||
*[[Beta-galactosidase assay]] | *[[Beta-galactosidase assay]] | ||
| - | + | ==External links== | |
| + | * S-Gal - a variation of X-Gal [http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Life_Science_Innovations/April_2001/April_2001_Feature_Article.html] | ||
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Current revision
X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for beta-galactosidase (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues.
A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation.
Contents |
Protocols using X-Gal
Notes
- Also may make stock solution with 50 mg in about 250 µl DMSO
See also
External links
- S-Gal - a variation of X-Gal [1]
