X-Gal: Difference between revisions
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X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening. | X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues. | ||
A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. | A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation. | ||
==Protocols using X-Gal== | ==Protocols using X-Gal== | ||
*[[LacZ staining of cells]] | *[[LacZ staining of cells]] | ||
==Notes== | |||
*Also may make stock solution with 50 mg in about 250 µl DMSO | |||
==See also== | ==See also== | ||
*[[Beta-galactosidase assay]] | *[[Beta-galactosidase assay]] | ||
==External links== | |||
* S-Gal - a variation of X-Gal [http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Life_Science_Innovations/April_2001/April_2001_Feature_Article.html] | |||
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Latest revision as of 14:22, 23 December 2011
X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for beta-galactosidase (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues.
A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation.
Protocols using X-Gal
Notes
- Also may make stock solution with 50 mg in about 250 µl DMSO
See also
External links
- S-Gal - a variation of X-Gal [1]
