X-Gal: Difference between revisions
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X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening. | X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for [[beta-galactosidase]] (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues. | ||
A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. | A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation. | ||
==Protocols using X-Gal== | ==Protocols using X-Gal== | ||
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==See also== | ==See also== | ||
*[[Beta-galactosidase assay]] | *[[Beta-galactosidase assay]] | ||
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Revision as of 08:38, 10 June 2008
X-Gal is the common short name for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside. It is a substrate for beta-galactosidase (lacZ) and turns blue upon being cleaved. It is commonly used for blue-white screening or detection of LacZ transgenes in cells and tissues.
A stock solution of X-gal is commonly made with 20 mg/ml X-gal in DMF (dimethylformamide). When pouring agar plates, a typical final concentration is 20 μg/ml. Note that DMF is toxic. Take care when preparing X-Gal solution. Protect X-Gal from light to prevent degradation.
