Wittrup: MOPS running buffer

Formulation

• 50mM MOPS
• 50mM Tris Base
• 0.1% SDS
• 1mM EDTA
• pH 7.7

1L of 1x Buffer

• 10.463g MOPS
• 6.057g Tris Base
• 1g SDS
• 0.292g EDTA
• pH to 7.7

5L of 1x Buffer

• 52.315g MOPS
• 30.285g Tris Base
• 5g SDS
• 1.461g EDTA
• pH to 7.7