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| Line 3: |
Line 3: |
| ==Calculations== | | ==Calculations== |
| :*[[WinterVomitingLab:Protocols/Virus_weight_calculations|Virus weight]] | | :*[[WinterVomitingLab:Protocols/Virus_weight_calculations|Virus weight]] |
|
| |
| What is the weight of human norovirus? Or VLPs?
| |
|
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| MW of ssRNA genome
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| = (7.5kb x 320.5) + 159.0
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| = 2,404 kDa (or g/ml)
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| = 3.99 x 10^-12 ug
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|
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| VP1 capsid of 90 dimers (180 copies)
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| = 60 kDa x 180
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| = 10,800 kDa
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| = 1.8 x 10^-11 ug
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|
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| VP2 (estimated 3 copies per capsid)
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| = 22 kDa x 3
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| = 66 kDa
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|
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| VPg (one copy per genome)
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| = 15.8 kDa
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|
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| For VLPs consisting of only VP1, see above.
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|
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| For viruses:
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| = 2,404 + 10,800 + 66 + 15.8
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| = 13,286 KDa
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| = 2.2 x 10^-11 ug
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|
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| Therefore, for a 1 ug/ml preparation of VLP,
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| = 1 / (1.8 x 10^-11)
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| = 5.6 x 10^10 viruses / ml
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|
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| For a virus stock that contains 1 x 10^7 viruses / ml
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| = (1 x 10^7 viruses) x (2.2 x 10^-11 ug)
| |
| = 0.00022 ug of viruses / ml
| |
