William A. C. Gendron Week 10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(More Definitions) |
(Cleaned up Definitions) |
||
Line 2: | Line 2: | ||
# Definitions | # Definitions | ||
#*Trehalose(also called mycose): This is "a non reducing disaccharide" which in this context it is being used as a cryoprotectant as it is brought to near freezing conditions. It can also act as a preservative and an anti-oxidant. TPS1 and TPS2 are responsible for its biosynthesis in yeast. Source: http://www.oxfordreference.com/view/10.1093/acref/9780198529170.001.0001/acref-9780198529170-e-19924?rskey=ZqLfPq&result=1 | #*Trehalose(also called mycose): This is "a non reducing disaccharide" which in this context it is being used as a cryoprotectant as it is brought to near freezing conditions. It can also act as a preservative and an anti-oxidant. TPS1 and TPS2 are responsible for its biosynthesis in yeast. Source: http://www.oxfordreference.com/view/10.1093/acref/9780198529170.001.0001/acref-9780198529170-e-19924?rskey=ZqLfPq&result=1 | ||
#*Mannoproteins: " | #*Mannoproteins: These are proteins "that contain 15 to 90% mannose by weight" Sources: http://link.springer.com/chapter/10.1007%2F978-3-642-68234-6_18 | ||
#*Desaturase: An enzyme that catalyzes desaturation. Desaturation is when "two hydrogen atoms or a hydrogen and a hydroxyl group are removed from adjacent atoms. Source: http://www.oxfordreference.com/view/10.1093/oi/authority.20110803095712459 http://www.oxfordreference.com/view/10.1093/acref/9780198529170.001.0001/acref-9780198529170-e-4993?rskey=k9Vxrx&result=1 | #*Desaturase: An enzyme that catalyzes desaturation. Desaturation is when "two hydrogen atoms or a hydrogen and a hydroxyl group are removed from adjacent atoms." Source: http://www.oxfordreference.com/view/10.1093/oi/authority.20110803095712459 http://www.oxfordreference.com/view/10.1093/acref/9780198529170.001.0001/acref-9780198529170-e-4993?rskey=k9Vxrx&result=1 | ||
#*Permease: | #*Permease: A membrane transport protein in or out of the cell. There really should not be two names for this. Source: http://www.oxfordreference.com/view/10.1093/oi/authority.20110803100318301 | ||
#*Zero trans-influx transport assay: I could not find an exact definition to how they do this but it measures the rates of glucose uptake in this context. Source: http://link.springer.com/article/10.1007/s00253-002-1085-6 | #*Zero trans-influx transport assay: I could not find an exact definition to how they do this but it measures the rates of glucose uptake in this context. None of the sources I found really defined how it was done even when I did a wider google search. Source: http://link.springer.com/article/10.1007/s00253-002-1085-6 | ||
#*Homeoviscous: | #*Homeoviscous: A cell will alter the number of long or saturated fatty acids in its cell membrane to adjust for the temperature. Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC388039/ | ||
#*DEAD Box Family: | #*DEAD Box Family: This refers to 4 amino acids that form in this order: -D-E-A-D- (Asp-Glu-Ala-Asp) and this acts as an ATP binding site. Source: http://www.oxfordreference.com/view/10.1093/oi/authority.20110803095704770?rskey=nZKYQA&result=2 | ||
#* | #*First-Order Kinetics: This is a chemical reaction that is only dependent on one of the reactants. Source: http://www.biology-online.org/dictionary/First-order_kinetics | ||
#* | #*Cis-regulatory: This is a sequence near the gene being referenced, which acts as a binding site for transcription factors. It may be in or near the gene. Source:http://www.bio.brandeis.edu/haberlab/jehsite/chIP.html | ||
#* | #*Chromatin immunoprecipitation: Chromatin immunoprecipitation, or ChIP, refers to a procedure used to determine whether a given protein binds to or is localized to a specific DNA sequence in vivo. The diagram below illustrates the basic steps of this procedure. Source: http://www.nature.com/scitable/definition/cis-regulatory-element-cis-regulatory-element-75 |
Revision as of 18:01, 23 March 2015
Paper Analysis- Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis
- Definitions
- Trehalose(also called mycose): This is "a non reducing disaccharide" which in this context it is being used as a cryoprotectant as it is brought to near freezing conditions. It can also act as a preservative and an anti-oxidant. TPS1 and TPS2 are responsible for its biosynthesis in yeast. Source: http://www.oxfordreference.com/view/10.1093/acref/9780198529170.001.0001/acref-9780198529170-e-19924?rskey=ZqLfPq&result=1
- Mannoproteins: These are proteins "that contain 15 to 90% mannose by weight" Sources: http://link.springer.com/chapter/10.1007%2F978-3-642-68234-6_18
- Desaturase: An enzyme that catalyzes desaturation. Desaturation is when "two hydrogen atoms or a hydrogen and a hydroxyl group are removed from adjacent atoms." Source: http://www.oxfordreference.com/view/10.1093/oi/authority.20110803095712459 http://www.oxfordreference.com/view/10.1093/acref/9780198529170.001.0001/acref-9780198529170-e-4993?rskey=k9Vxrx&result=1
- Permease: A membrane transport protein in or out of the cell. There really should not be two names for this. Source: http://www.oxfordreference.com/view/10.1093/oi/authority.20110803100318301
- Zero trans-influx transport assay: I could not find an exact definition to how they do this but it measures the rates of glucose uptake in this context. None of the sources I found really defined how it was done even when I did a wider google search. Source: http://link.springer.com/article/10.1007/s00253-002-1085-6
- Homeoviscous: A cell will alter the number of long or saturated fatty acids in its cell membrane to adjust for the temperature. Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC388039/
- DEAD Box Family: This refers to 4 amino acids that form in this order: -D-E-A-D- (Asp-Glu-Ala-Asp) and this acts as an ATP binding site. Source: http://www.oxfordreference.com/view/10.1093/oi/authority.20110803095704770?rskey=nZKYQA&result=2
- First-Order Kinetics: This is a chemical reaction that is only dependent on one of the reactants. Source: http://www.biology-online.org/dictionary/First-order_kinetics
- Cis-regulatory: This is a sequence near the gene being referenced, which acts as a binding site for transcription factors. It may be in or near the gene. Source:http://www.bio.brandeis.edu/haberlab/jehsite/chIP.html
- Chromatin immunoprecipitation: Chromatin immunoprecipitation, or ChIP, refers to a procedure used to determine whether a given protein binds to or is localized to a specific DNA sequence in vivo. The diagram below illustrates the basic steps of this procedure. Source: http://www.nature.com/scitable/definition/cis-regulatory-element-cis-regulatory-element-75