Wikiomics:Site Directed Mutagenesis

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##if not check out [http://speedy.embl-heidelberg.de/gtsp/flowchart2.html  STRUCTURE PREDICTION FLOWCHART]
##if not check out [http://speedy.embl-heidelberg.de/gtsp/flowchart2.html  STRUCTURE PREDICTION FLOWCHART]
#What are the known domains inside our protein ?  
#What are the known domains inside our protein ?  
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## see [[Searching for 3D functional sites in a protein structure]]
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## see [[Wikiomics:Searching for 3D functional sites in a protein structure]]
## Where is the Active Site ? ([http://bioinfo2.weizmann.ac.il/~pietro/SARIG/V3/index.html SARIG])
## Where is the Active Site ? ([http://bioinfo2.weizmann.ac.il/~pietro/SARIG/V3/index.html SARIG])
## What is the location of the mutation? (surface / inside the protein)  
## What is the location of the mutation? (surface / inside the protein)  

Revision as of 01:29, 20 November 2007

This page is beta. please feel free to update it.

The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein.

  1. First step is to choose your target. (This is up to you).
  2. Finding the nucleic sequence (ENTREZ)
  3. What type of mutation will be introduced ? (substitution / deletion / addition)
  4. Can we find the wanted mutation in other related proteins ? (Blast)
  5. How conserved is the protein we wish to mutate ?
  6. Does the w.t. protein has a known 3D structure ? (Check PDB)
    1. if not check out STRUCTURE PREDICTION FLOWCHART
  7. What are the known domains inside our protein ?
    1. see Wikiomics:Searching for 3D functional sites in a protein structure
    2. Where is the Active Site ? (SARIG)
    3. What is the location of the mutation? (surface / inside the protein)
  8. What impact does the mutation have on the protein (no backbone changes yet)
  9. If the protein is a multiple subunit protein - what are the subunit interaction residues ? (CSU)
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