Wikiomics:PSI-BLAST - Revision history

Torsten Waldminghaus: added tags

Torsten Waldminghaus — Mon, 03 Mar 2008 21:35:31 GMT

added tags

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	* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi3.html @NCBI Part 3]		* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi3.html @NCBI Part 3]
	* [http://gepard.bioinformatik.uni-saarland.de/html/SoftwarewerkzeugeWS0304-Dateien/Praktikum-BLAST-eng.doc @Uni-Saaraland (DOC)]]		* [http://gepard.bioinformatik.uni-saarland.de/html/SoftwarewerkzeugeWS0304-Dateien/Praktikum-BLAST-eng.doc @Uni-Saaraland (DOC)]]
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		+	[[Category:Protocol]] [[Category:In silico]] [[Category:Sequence analysis]]

Bill Flanagan: 1 revision(s)

Bill Flanagan — Tue, 20 Nov 2007 04:00:47 GMT

1 revision(s)

←Older revision

Revision as of 04:00, 20 November 2007

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Darked — Fri, 23 Feb 2007 15:49:27 GMT

lit

New page

'''PSI-BLAST''' - Position-Specific Iterated BLAST. Variant of [[BLAST]] used for disovery of weak but relevant protein sequence matches. The algorithm is based on running consequitive BLAST searches using position weight matices:

* query sequence ->
* first blast search ->
* first iteration blast results ->
* sequence alignment of strong matches ->
* creating a profile #1 ->
* second iteration blast search with profile #1 as a query ->
* second iteration blast results, etc.

PSI-BLAST server at NCBI: [http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi?CMD=Web&LAYOUT=TwoWindows&AUTO_FORMAT=Semiauto&ALIGNMENTS=250&ALIGNMENT_VIEW=Pairwise&CLIENT=web&COMPOSITION_BASED_STATISTICS=on&DATABASE=nr&CDD_SEARCH=on&DESCRIPTIONS=500&ENTREZ_QUERY=%28none%29&EXPECT=10&FORMAT_OBJECT=Alignment&FORMAT_TYPE=HTML&I_THRESH=0.005&MATRIX_NAME=BLOSUM62&NCBI_GI=on&PAGE=Proteins&PROGRAM=blastp&RUN_PSIBLAST=on&SERVICE=plain&SET_DEFAULTS.x=36&SET_DEFAULTS.y=5&SHOW_OVERVIEW=on&END_OF_HTTPGET=Yes&SHOW_LINKOUT=yes&GET_SEQUENCE=yes&SEARCH_NAME=psi_bp link]

==Tutorials==
* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi1.html @NCBI Part 1]
* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi2a.html @NCBI Part 2]
* [http://www.ncbi.nlm.nih.gov/Education/BLASTinfo/psi3.html @NCBI Part 3]
* [http://gepard.bioinformatik.uni-saarland.de/html/SoftwarewerkzeugeWS0304-Dateien/Praktikum-BLAST-eng.doc @Uni-Saaraland (DOC)]]