Wikiomics:Nuclear Magnetic Resonance

Biological Nuclear Magnetic Resonance or NMR is a technique which allows the determination of full or partial 3-dimensional structures of macromolecules. As opposed to Wikiomics:X-ray crystallography, NMR does not require the molecules to be arranged periodically in a crystal and therefore, mixture of molecules in different conformations can be studied dynamically.

Basics of NMR

NMR can detect transition of nuclear spins. The most useful nuclei are those with nuclear spin 1/2. Many elements have such isotopes. Some are naturally abundant (1H) but most are not (13C, 15N)

Isotopic Labeling

In order to perform most modern experiments, it is necessary to produce, isotopically enriched proteins. This is achieved by heterologous expression in Minimal Media. In this case, the nitrogen and carbon sources are controlled and the desired isotopic enrichment can be achieved. Selective labeling of proteins is more complicated.

Sample preparation

NMR suffers from low sensitivity so the samples have to be sufficiently concentrated. Usually 500ul of 1mM sample is optimal for collecting all the needed experiments, however, with the current developments in Instrumentation (See crycrobes and nanoprobes) it is possible to work with a few mg of protein

See also

• Wikipedia:NMR gives an introduction to NMR in general (but not much about its use in Wikiomics:Structural biology)
• Wikiomics:Protein Data Bank
• BioMagResBank is a repository of NMR data from which 3D models are derived and stored into the Wikiomics:PDB.