Wikiomics:BLAST tutorial (exercises)

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  • blastp with shuffled sequence: note that all hits must be wrong! 
>Shuffled_protein
MNALFNGRYVRQNLRFAGFARVDRERPPLTWFQVSAIPLFARGFAGAIDSSRISRSGEAF
VGCVCLAQPCFVGAADGEADLRAGVGSVTVAVNGSLLLFALG
  • what is this nucleotide sequence? 
>nucleotide_1
ATATAGTTTTTAATTAGGAGGATTTTATCAATGAAAATATCAATACTATACAGTAGCAAGACAGGAAAAA
CCGAAAGAGTTGCCAAGTTAATTGAAGAAGGTGTGAAGAGGTCTGGAAATATTGAAGTAAAAACTATGAA
TTTAGATGCAGTTGATAAAAAGTTCTTACAAGAATCAGAAGGAATTATTTTTGGAACACCAACTTATTAT
  • same sequence mutated: 
>nucleotide_2
ATATAGTATTTAATTAGGAGGATTTCATCAATGACACTCTCAATACTATACAGTAGCAAG
ACAGGGAAAACAGAAAGAGTTGCCAAGGTAATTGAGGAAGGTGTGAAGAGGTCTCAAAAT
AATGAAGTAAGAACTGTGAATCTAGATGCAGTTCATAATAAGTTCTTCGAAGAATCAGGA
GGCATTCTTTTTGGAACACCAACTTATGAT
  • what is it? Any remote similarity? 
>protein_1
HIGSDTDGDDDSETEGEWNLKEGGSIDAELSHSRKAKKVESLLRKLHTKDILPEEVSS
GSHLHTRSPMVLFLSLAVPTDKVVMFYKALFTEAELKAPLESLKRFQNNGDARTGKSA
IFMLGGGHFAGAVISHEPVKVKGQLLNEELPYLQKIQSINVLESKTFHRYTTRRKQGG
  • play with your favorite gene/protein
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