Western Blots

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Revision as of 09:05, 31 August 2006 by Crp724 (talk | contribs) (→‎Procedure)
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SDS-PAGE Westerns

Materials

  • 2x PLS
  • PBS-T
  • 5% milk in PBS-T

Procedure

Prepare Samples

  • Boil Samples for 5 min, cool on ice 1 min, spin 1 min
  • Ladders: 8ul ladder + 8ul 2x PLS
    • High mol weight 30KDa - 220 KDa
    • Low mol weight 6.5KDa - 45 Kda
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