Weiss Lab:TrafficPaper

Additional data for

Dynamic Partitioning of a GPI-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking

Fabien Pinaud[1,3], Xavier Michalet[1,3], Gopal Iyer[1], Emmanuel Margeat[1], Hsiao-Ping Moore[2], Shimon Weiss[1,3].

[1]Department of Chemistry & Biochemistry, University of California, Los Angeles, CA, USA.

[2]Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA.

[3]Corresponding authors: fabien.pinaud@lkb.ens.fr; michalet@chem.ucla.edu and sweiss@chem.ucla.edu

Published in Traffic

Note: The following additional data are part of the supplementary information that were submitted and reviewed for publication of the manuscript in Traffic. Unfortunately, because of size constrains, these additional data could not be posted together with the rest of the supplementary information on Traffic website.

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These data include:

Single-molecule dual-color total internal reflection fluorescence (TIRF) microscopy setups

Crosslinking of Av-GPI induces patches in the absence of CTxB

Comparison between single-trajectory PDSD analysis and global PDSD analysis

Distribution of Av-GPI diffusion coefficients in untreated HeLa cells at 37°C

Visualization of slow/immobile molecules using mean intensity projection images

Immunostaining of endogenous caveolin-1 and colocalization with caveolin-1-EGFP in two HeLa cell lines

Quantification of Av-GPI membrane expression in Av-GPI and Av-GPI/Cav1-EGFP expressing HeLa cells

Diffusion coefficients and confinement sizes of Av-GPI for various treatments of HeLa cells at room temperature